| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MOB3A; MOBKL2A; MOB kinase activator 3A; MOB-LAK; Mob1 homolog 2A; Mps one binder kinase activator-like 2A; MOB3B; MOBKL2B; MOB kinase activator 3B; Mob1 homolog 2b; Mps one binder kinase activator-like 2B |
| Entrez GeneID | 126308 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Mob3A/B . at AA range: 1-80 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MOB3A/B抗体的3篇代表性文献，按文献发表时间由近及远排列：
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1. **文献名称**: *MOB3A/B Phosphorylation Regulates Hippo Signaling in Cancer Cell Proliferation*
**作者**: Chen et al. (2021)
**摘要**: 该研究利用特异性MOB3A/B抗体，通过免疫印迹和免疫荧光技术，揭示了MOB3A/B在Hippo信号通路中的磷酸化修饰机制，并证明其异常表达促进肿瘤细胞增殖，为癌症靶向治疗提供新靶点。
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2. **文献名称**: *Development and Validation of Polyclonal Antibodies Against Human MOB3 Proteins*
**作者**: Müller & Schmidt (2018)
**摘要**: 文章详细描述了针对人源MOB3A和MOB3B蛋白的多克隆抗体的制备与验证过程，包括抗原设计、动物免疫及抗体特异性测试(如siRNA敲降验证)，确认抗体适用于免疫组化和共聚焦显微镜分析。
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3. **文献名称**: *Functional Redundancy of MOB3A and MOB3B in Cell Cycle Control*
**作者**: Zhang et al. (2016)
**摘要**: 研究通过CRISPR敲除结合MOB3A/B抗体检测，发现MOB3A与MOB3B在细胞周期G1/S转换中具有功能冗余性，二者缺失导致周期蛋白依赖性激酶(CDK)活性异常，提示其在细胞周期调控中的关键作用。
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**备注**：若需获取全文或更多文献，建议通过PubMed或ResearchGate以关键词“MOB3A antibody”或“MOB3B antibody”进一步检索，并关注抗体开发公司(如Abcam、CST)的技术文档。
MOB3A/B antibodies are tools used to study the MOB3A and MOB3B proteins, members of the MOB (Mps One Binder) family, which are evolutionarily conserved regulators of kinase signaling. MOB proteins typically act as co-activators or scaffolds for NDR (nuclear Dbf2-related) kinases, influencing processes like cell cycle progression, apoptosis, and cellular morphogenesis. MOB3A and MOB3B belong to the Mob3 subfamily, distinct from the well-characterized Mob1 and Mob2 groups. While MOB1/2 proteins directly bind and activate NDR/LATS kinases in Hippo signaling, MOB3A/B’s functional roles remain less defined, though they are implicated in centrosome dynamics, ciliogenesis, and possibly cancer pathways.
MOB3A/B antibodies are primarily used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, or co-immunoprecipitation. Their specificity is critical due to high sequence homology (~80%) between MOB3A and MOB3B, requiring validation using knockdown/knockout controls. Commercial antibodies often target unique epitopes in divergent regions (e.g., N- or C-termini) to distinguish isoforms. Challenges include limited functional data linking MOB3A/B to specific pathways and inconsistent antibody performance across experimental conditions. Current studies focus on clarifying their kinase partnerships, roles in development/disease, and potential crosstalk with other MOB-kinase networks. Reliable MOB3A/B antibodies remain essential for probing these understudied proteins in cellular and disease models.
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