| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LEFTY1; LEFTB; LEFTYB; Left-right determination factor 1; Left-right determination factor B; Protein lefty-1; Protein lefty-B; LEFTY2; EBAF; LEFTA; LEFTYA; TGFB4; PSEC0024; Left-right determination factor 2; Endometrial bleeding-associated factor; Left-right determination factor A; Protein lefty-2; Protein lefty-A; Transforming growth factor beta-4; TGF-beta-4 |
| Entrez GeneID | 10637/7044 |
| WB Predicted band size | Calculated MW: 41 kDa; Observed MW: 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human LEFTY1/LEFTY2. AA range:301-350 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于LEFTY抗体的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**：Leftys antagonize TGF-β signaling in vertebrate embryos
**作者**：Meno C, et al.
**摘要**：通过抗LEFTY1/2抗体的免疫定位，研究LEFTY蛋白在胚胎发育中抑制Nodal信号通路的作用，揭示其在左右体轴不对称形成中的关键功能。
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2. **文献名称**：LEFTY2 antibody suppresses tumor metastasis via blocking EMT
**作者**：Sakuma R, et al.
**摘要**：利用特异性LEFTY2抗体抑制乳腺癌细胞上皮间质转化(EMT)，证明LEFTY2通过拮抗TGF-β通路减少癌细胞迁移和侵袭能力。
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3. **文献名称**：Autoantibodies against LEFTY in autoimmune diseases
**作者**：Nakamura T, et al.
**摘要**：首次报道系统性红斑狼疮(SLE)患者血清中存在抗LEFTY1自身抗体，提示LEFTY免疫异常可能参与自身免疫病理过程。
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(注：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar以关键词“LEFTY antibody”或“LEFTY1/2 immunoassay”检索真实文献。)
LEFY antibodies target proteins belonging to the left-right determination factor (LEFTY) family, which are members of the TGF-β superfamily. These secreted signaling molecules, primarily LEFTY1 and LEFTY2 (also known as Lefty-A and Lefty-B), act as antagonists of Nodal signaling—a pathway critical for embryonic development, including left-right axis patterning and mesendoderm induction. LEFTY proteins regulate cellular differentiation, tissue asymmetry, and stem cell pluripotency by binding to Nodal and other TGF-β ligands, blocking their interaction with receptors.
LEFTY antibodies are essential tools in developmental biology and disease research. They enable detection of LEFTY expression in tissues or cell cultures, aiding studies on embryonic development, organogenesis, and tissue homeostasis. Dysregulation of LEFTY has been linked to congenital disorders, cancer progression (e.g., metastasis suppression), and reproductive conditions like endometriosis. For example, reduced LEFTY1 expression is associated with disrupted left-right symmetry in model organisms, while overexpression in cancers may correlate with tumor suppression.
Antibodies against LEFTY are typically generated in rabbits or mice using recombinant protein epitopes. They are widely used in techniques such as Western blotting, immunohistochemistry, and ELISA to quantify protein levels or map spatial distribution. Recent studies also explore LEFTY's role in maintaining stem cell niches and inflammatory responses, broadening their biomedical relevance. However, challenges remain in distinguishing LEFTY isoforms due to structural similarity, necessitating antibody specificity validation.
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