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Rabbit Polyclonal KLF12 Antibody

  • 中文名： KLF12抗体
  • 别    名： KLF12; AP2REP; HSPC122; Krueppel-like factor 12; Transcriptional repressor AP-2rep
货号: IPDX23306
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesKLF12; AP2REP; HSPC122; Krueppel-like factor 12; Transcriptional repressor AP-2rep
Entrez GeneID11278
WB Predicted band sizeCalculated MW: 44 kDa; Observed MW: 44 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenSynthesized peptide derived from the Internal region of human KLF12.
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于KLF12抗体的3篇参考文献示例(注：文献信息为模拟概括，实际引用请核实原文)：

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1. **文献名称**：*KLF12 Suppresses Gastric Cancer Progression by Regulating Cell Invasion and Apoptosis*

**作者**：Chen L, et al.

**摘要**：本研究利用KLF12特异性抗体进行Western blot和免疫组化分析，发现KLF12在胃癌组织中表达下调，其通过抑制MMP9通路减少癌细胞侵袭并促进凋亡，提示其作为抑癌因子的潜在作用。

2. **文献名称**：*KLF12-Mediated Transcriptional Repression in Epithelial-Mesenchymal Transition*

**作者**：Smith JR, et al.

**摘要**：通过KLF12抗体的染色质免疫沉淀(ChIP)技术，揭示KLF12直接结合E-cadherin启动子区域抑制其表达，促进乳腺癌细胞上皮间质转化(EMT)，为转移机制提供新见解。

3. **文献名称**：*Developmental Expression of KLF12 in Mouse Embryogenesis*

**作者**：Wang Y, et al.

**摘要**：采用KLF12抗体对小鼠胚胎进行免疫荧光染色，发现KLF12在神经管和肢芽发育阶段高表达，提示其在胚胎形态发生和细胞分化中的调控功能。

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如需实际文献，建议通过PubMed或Google Scholar检索关键词“KLF12 antibody”或“KLF12 function”获取具体研究。

背景信息

The Krüppel-like factor 12 (KLF12) is a member of the Krüppel-like factor family of transcription factors, characterized by conserved C-terminal zinc finger domains that mediate DNA binding. KLF12 plays roles in regulating gene expression involved in cell proliferation, differentiation, and apoptosis. It is broadly expressed in tissues, including the brain, heart, and reproductive organs, and has been implicated in developmental processes, cancer progression (e.g., gastric, breast, and ovarian cancers), and metabolic disorders. KLF12 antibodies are immunological tools designed to detect and quantify KLF12 protein levels in experimental settings. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Due to KLF12's functional overlap with other KLF family members, high specificity is critical, often achieved through epitope mapping and validation using knockout cell lines or siRNA-mediated knockdown. Commercially available KLF12 antibodies are typically raised in rabbits or mice, with formats including monoclonal and polyclonal variants. Researchers utilize these antibodies to explore KLF12's regulatory mechanisms, such as its interaction with AP-2α or its role in repressing target genes. Proper validation, including positive/negative controls and molecular weight confirmation, is essential to ensure reliable experimental outcomes.

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