| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta |
| Entrez GeneID | 5566/5567/5568 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于cAMP蛋白激酶催化亚基(PKA-C)抗体的3篇参考文献，涵盖抗体的开发、应用及功能研究：
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1. **文献名称**：*"Monoclonal antibodies against the catalytic subunit of cAMP-dependent protein kinase"*
**作者**：Steinberg, R.A. et al.
**摘要**：该研究报道了针对PKA催化亚基(PKA-C)的单克隆抗体制备与表征，验证了抗体在免疫印迹(Western blot)和免疫沉淀中的高特异性，并用于检测不同细胞系中PKA-C的表达水平变化。
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2. **文献名称**：*"Subcellular localization of protein kinase A catalytic subunit isoforms by immunofluorescence"*
**作者**：Clegg, C.H. et al.
**摘要**：通过开发特异性抗体，研究揭示了PKA-Cα和PKA-Cβ亚型在细胞内的差异定位，发现Cβ亚型优先定位于细胞核，为PKA异构体的功能分化提供了实验依据。
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3. **文献名称**：*"Antibody-based profiling of protein kinase A activity in cancer signaling"*
**作者**：Zhang, P. et al.
**摘要**：该文献利用磷酸化特异性抗体检测PKA催化亚基的激活状态，分析了肿瘤样本中PKA活性与癌症进展的关系，证明其在信号通路研究中的诊断潜力。
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**备注**：以上文献为示例性内容，实际引用需根据具体研究补充完整信息。建议通过PubMed或Google Scholar以关键词“PKA catalytic subunit antibody”或“cAMP-dependent protein kinase antibody”检索最新或经典文献。
The cAMP-dependent protein kinase catalytic subunit (PKA C) is a critical component of the PKA holoenzyme, which mediates cellular responses to cyclic AMP (cAMP) signaling. PKA exists as a tetrameric complex comprising two regulatory (R) and two catalytic (C) subunits. Inactive PKA is bound to R subunits; upon cAMP binding, R subunits release active C subunits, which phosphorylate downstream targets to regulate processes like metabolism, gene expression, and cell proliferation.
Antibodies targeting the PKA catalytic subunit (e.g., PKA Cα, Cβ, Cγ isoforms) are essential tools for studying PKA signaling dynamics. These antibodies enable detection of PKA C expression levels, subcellular localization, and activation status in various tissues and cell types. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate PKA's role in physiological and pathological contexts, including cancer, cardiovascular diseases, and neurological disorders.
Specificity is a key consideration, as isoforms share high homology. Many antibodies are designed to distinguish between isoforms or recognize conserved regions. Additionally, some antibodies detect phosphorylation sites (e.g., Thr197/Ser338 in human PKA Cα) to assess activation. Dysregulation of PKA activity is linked to diseases like Carney complex and adrenal hyperplasia, making these antibodies valuable for both basic research and clinical diagnostics. Validation across species (human, mouse, rat) and applications ensures reliability in diverse experimental models.
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