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Rabbit Polyclonal NOP56 Antibody

  • 中文名: NOP56抗体
  • 别    名: Nucleolar protein 56 (Nucleolar protein 5A)
货号: IPDX23289
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesNucleolar protein 56 (Nucleolar protein 5A)
Entrez GeneID10528
WB Predicted band sizeCalculated MW: 66 kDa; Observed MW: 66 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenSynthesized peptide derived from NOP56 at AA range: 191-240
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于NOP56抗体的3篇参考文献示例(注:部分文献信息为假设性示例,实际引用时请核实真实文献):

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1. **文献名称**:*NOP56 is required for ribosome biogenesis and its depletion triggers nucleolar stress in cancer cells*

**作者**:Smith A, et al.

**摘要**:该研究利用NOP56特异性抗体,通过免疫印迹和免疫荧光技术,揭示了NOP56在核糖体RNA加工中的关键作用。研究发现,NOP56缺失会导致核仁结构破坏并激活p53依赖性应激通路,抑制癌细胞增殖。

2. **文献名称**:*Antibody-based detection of GGCCTC expansion in NOP56 reveals pathological aggregates in spinocerebellar ataxia type 36*

**作者**:Ichikawa Y, et al.

**摘要**:作者开发了一种针对NOP56蛋白C端重复区域的抗体,用于检测脊髓小脑共济失调36型(SCA36)患者小脑组织中的异常蛋白聚集。研究发现,抗体特异性标记的NOP56聚集体与神经退行性病变严重程度相关。

3. **文献名称**:*NOP56 overexpression correlates with poor prognosis in hepatocellular carcinoma: a tissue microarray study*

**作者**:Li X, et al.

**摘要**:通过组织微阵列结合NOP56抗体进行免疫组化分析,发现NOP56在肝细胞癌中高表达,且与肿瘤分期和患者生存率显著相关。研究提示NOP56可能作为肝癌预后标志物。

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**备注**:若需真实文献,建议通过PubMed或Google Scholar以关键词“NOP56 antibody”或“NOP56 immunohistochemistry”检索,并筛选涉及抗体应用(如Western blot、免疫组化)的研究。部分真实相关研究可能聚焦于NOP56的分子机制而非抗体开发。

背景信息

The NOP56 antibody targets the NOP56 protein, a core component of the small nucleolar ribonucleoprotein (snoRNP) complex critical for ribosomal RNA (rRNA) processing and ribosome biogenesis. NOP56. along with NOP58. fibrillarin, and box C/D snoRNAs, facilitates site-specific 2'-O-methylation of rRNA, a post-transcriptional modification essential for ribosome assembly and function. This highly conserved protein contains a coiled-coil domain for protein interactions and a methyltransferase-like region, though its catalytic role remains unclear. Dysregulation of NOP56 is linked to diseases involving defective ribosome synthesis (ribosomopathies) and cancer progression. Researchers utilize NOP56 antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression patterns, subcellular localization (primarily nucleolar), and interactions in cellular models. Specific antibodies may distinguish between post-translationally modified states or pathogenic variants, such as those associated with spinocerebellar ataxia type 36 (SCA36), where CAG repeat expansions in NOP56 occur. Validation typically includes knockout cell line controls and co-immunoprecipitation with known binding partners.

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