| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PREP; PEP; Prolyl endopeptidase; PE; Post-proline cleaving enzyme |
| Entrez GeneID | 5550 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from PREP . at AA range: 10-90 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PREP(脯氨酰寡肽酶)抗体的3篇参考文献示例，格式为文献名称、作者及摘要概括：
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1. **"Development of a Specific Monoclonal Antibody for Prolyl Endopeptidase (PREP) and Its Application in Neurodegenerative Disease Models"**
*Authors: Smith J, et al.*
**摘要**：本研究开发了一种高特异性的抗人PREP单克隆抗体，并通过免疫印迹和免疫组化验证其在脑组织中的反应性。实验表明，该抗体能有效检测阿尔茨海默病模型中PREP的表达上调，提示其在神经炎症中的作用。
2. **"Prolyl Endopeptidase Inhibition Attenuates Cognitive Deficits via Antibody-Based Targeting in Transgenic Mice"**
*Authors: Tanaka K, et al.*
**摘要**：通过使用新型抗PREP抗体，研究者发现抑制PREP活性可减少tau蛋白过度磷酸化，改善转基因小鼠的认知功能。抗体被用于脑脊液和皮层组织的PREP定量分析，证实其诊断和治疗潜力。
3. **"Subcellular Localization of PREP in Neuronal Cells: Insights from Immunofluorescence Studies"**
*Authors: Müller R, et al.*
**摘要**：利用多克隆抗PREP抗体结合共聚焦显微镜，本研究揭示了PREP在神经元中的亚细胞分布(主要富集于胞质溶胶)，并发现其在突触功能调控中的潜在机制。
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**说明**：以上文献为示例，实际文献需通过PubMed或Web of Science等平台检索关键词(如“Prolyl endopeptidase antibody”“PREP immunoassay”)获取。建议结合具体研究方向筛选近年高被引论文。
PREP (prolyl endopeptidase), also known as prolyl oligopeptidase or POP, is a serine protease that hydrolyzes peptides shorter than 30 amino acids at proline residues. Initially identified in the 1970s, it is implicated in neuropeptide metabolism, inositol signaling, and protein processing. PREP is predominantly cytosolic and expressed in various tissues, including the brain, where it may influence cognitive functions and neurodegenerative processes. Studies link PREP dysregulation to Alzheimer’s disease, Parkinson’s disease, and other neurological disorders, potentially through interactions with α-synuclein or tau protein aggregation.
Antibodies targeting PREP are essential tools for investigating its expression, localization, and function. These antibodies are typically raised against specific epitopes of human or murine PREP and validated via techniques like Western blot, immunohistochemistry, or knockout validation. Applications range from basic research exploring PREP's role in neurodegeneration to drug development screening for PREP inhibitors. Some antibodies distinguish between full-length PREP and its cleavage products, aiding studies on its activation or proteolytic activity. Challenges include ensuring specificity due to structural similarities with other proteases. Recent advances in antibody engineering have improved selectivity, supporting ongoing efforts to clarify PREP's pathophysiological roles and therapeutic potential.
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