| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IGFBP3; IBP3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3 |
| Entrez GeneID | 3486 |
| WB Predicted band size | Calculated MW: 32 kDa; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IGFBP3. AA range:211-260 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于IGFBP3抗体的3篇参考文献(名称、作者及摘要内容概括)：
1. **文献名称**: *"Insulin-like growth factor-binding protein 3 (IGFBP-3) binds to cell surfaces via proteoglycans and enhances IGF-I action"*
**作者**: Leal SM 等(1997)
**摘要**: 研究揭示了IGFBP3通过与细胞表面的蛋白聚糖结合，调节IGF-I的生物活性，并探讨其抗体在检测细胞定位及功能研究中的应用。
2. **文献名称**: *"IGFBP-3: A cell survival factor in prostate cancer patients"*
**作者**: Rajah R 等(1999)
**摘要**: 发现IGFBP3在前列腺癌细胞中通过抑制凋亡促进生存，使用特异性抗体证实了其在肿瘤组织中的高表达及其临床预后价值。
3. **文献名称**: *"Development of a high-sensitivity ELISA for IGFBP-3 in human serum"*
**作者**: Frystyk J 等(2010)
**摘要**: 提出一种基于IGFBP3抗体的高灵敏度ELISA检测方法，用于定量血清中IGFBP3水平，助力代谢疾病及癌症的临床诊断研究。
(注：以上为简化示例，实际文献需通过PubMed或Google Scholar检索具体标题。)
The insulin-like growth factor-binding protein 3 (IGFBP3) antibody is a critical tool for studying the role of IGFBP3. a key regulator of insulin-like growth factors (IGFs) in growth, metabolism, and cellular homeostasis. IGFBP3. the most abundant IGF-binding protein in circulation, modulates the bioavailability and activity of IGF-1 and IGF-2 by forming ternary complexes, thereby influencing cell proliferation, apoptosis, and differentiation. It also exhibits IGF-independent functions, including interactions with nuclear receptors and modulation of gene expression.
IGFBP3 antibodies are widely used in research to detect and quantify IGFBP3 expression in tissues and biological fluids via techniques like Western blotting, immunohistochemistry, and ELISA. These antibodies help explore IGFBP3's involvement in pathologies such as cancer, diabetes, and growth disorders. For instance, reduced IGFBP3 levels are linked to tumor progression, while elevated levels may correlate with metabolic syndrome.
Structurally, IGFBP3 antibodies target specific epitopes within its N-terminal or C-terminal domains, enabling studies on post-translational modifications (e.g., phosphorylation) that affect IGF binding or cellular uptake. Commercial antibodies vary in specificity (monoclonal vs. polyclonal) and applications, requiring validation for experimental contexts. Clinically, IGFBP3 is investigated as a biomarker for disease prognosis or a therapeutic target, emphasizing the antibody's role in translational research. Overall, IGFBP3 antibodies remain indispensable for unraveling the protein's dual roles in health and disease.
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