| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Probable G-protein coupled receptor 75 |
| Entrez GeneID | 10936 |
| WB Predicted band size | Calculated MW: 59 kDa; Observed MW: 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GPR75. AA range:381-430 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于GPR75抗体的3篇参考文献示例(注：部分文献信息为模拟示例，实际引用时请核实具体文献)：
1. **文献名称**: *GPR75 deletion protects against obesity and metabolic disease in mice*
**作者**: Lopes, R. et al.
**摘要**: 本研究通过基因敲除技术发现GPR75缺失可显著减少高脂饮食诱导的小鼠肥胖，并改善胰岛素敏感性。研究使用特异性GPR75抗体验证了受体在脂肪组织和下丘脑中的表达，提示其可能通过调控能量代谢通路发挥作用。
2. **文献名称**: *Development and validation of a GPR75-specific monoclonal antibody for functional studies*
**作者**: Patel, S. et al.
**摘要**: 该文献报道了一种高亲和力、高特异性的GPR75单克隆抗体的开发，并通过流式细胞术、免疫荧光和Western blot验证其应用。该抗体成功用于检测GPR75在不同细胞系中的表达定位及配体结合后的构象变化。
3. **文献名称**: *GPR75 signaling in energy homeostasis: Insights from antibody-mediated receptor blockade*
**作者**: Zhang, M. et al.
**摘要**: 研究利用抗GPR75的中和抗体阻断受体活性，发现其可抑制神经元中与食欲调控相关的信号通路(如AMPK/mTOR)，表明GPR75可能通过中枢神经系统调节摄食行为和能量消耗。
4. **文献名称**: *GPR75 as a therapeutic target in metabolic disorders: Antibody-based intervention studies*
**作者**: Johnson, K. et al.
**摘要**: 通过动物模型验证抗GPR75抗体在治疗肥胖和糖尿病中的潜力。研究发现，抗体处理后的小鼠体重增加减缓，脂肪炎症减少，提示靶向GPR75的抗体可能成为代谢性疾病的新型治疗策略。
(提示：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等平台检索最新文献。)
**Background of GPR75 Antibody**
GPR75 (G Protein-Coupled Receptor 75) is an orphan receptor belonging to the G protein-coupled receptor (GPCR) family, which plays roles in cellular signaling and metabolic regulation. Although its endogenous ligand remains unidentified, GPR75 has been implicated in energy homeostasis, neuroendocrine functions, and pathologies such as obesity and diabetes. Studies suggest it interacts with chemokines or neuropeptides, potentially influencing appetite, insulin secretion, or adipose tissue activity.
The GPR75 antibody is a critical tool for detecting and studying this receptor’s expression, localization, and function. It is widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to explore GPR75’s role in tissues, particularly the brain, pancreas, and adipose depots. Recent research highlights its relevance in metabolic disorders; for instance, genetic variants of GPR75 are linked to obesity risk, with loss-of-function mutations associated with reduced body weight in humans (Nature 2021).
Antibodies targeting GPR75 also aid in drug discovery, enabling the validation of receptor targeting in preclinical models. However, challenges persist due to GPR75’s orphan status and overlapping signaling pathways with other GPCRs. Ongoing studies aim to clarify its ligand interactions and therapeutic potential, positioning GPR75 antibodies as vital reagents in both basic and translational research.
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