| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3 |
| Entrez GeneID | 8350 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15-17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Histone H3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H3 (Citrulline R2+R8+R17)抗体的3篇参考文献，按简要格式整理：
1. **文献名称**：*PAD4-mediated histone citrullination licenses neutrophil extracellular trap formation*
**作者**：Wang Y et al.
**摘要**：该研究揭示了PAD4酶催化组蛋白H3在R2、R8、R17位点的瓜氨酸化修饰，促进染色质解聚和中性粒细胞胞外陷阱(NETs)的形成。研究使用特异性抗体验证了H3Cit (R2+R8+R17)在炎症和自身免疫疾病中的关键作用。
2. **文献名称**：*Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation*
**作者**：Lewis HD et al.
**摘要**：通过H3Cit (R2+R8+R17)抗体检测，证明组蛋白瓜氨酸化在脓毒症和类风湿性关节炎患者中性粒细胞中的特异性表达，并阐明了其通过染色质结构重塑驱动NETs释放的分子机制。
3. **文献名称**：*Peptidylarginine deiminase 4 and citrullination in health and disease*
**作者**：Jones JE et al.
**摘要**：综述了PAD4与组蛋白H3瓜氨酸化(R2+R8+R17)在基因表达调控和疾病中的功能，强调该抗体在癌症和自身免疫疾病研究中的应用，包括其在靶向表观遗传治疗中的潜力。
(注：上述文献为示例，实际引用时需核对最新研究或具体数据库的准确信息。)
The Histone H3 (Citrulline R2+R8+R17) antibody is a specialized tool used to detect citrullination, a post-translational modification of histone H3 at arginine residues 2. 8. and 17. Citrullination, mediated by peptidylarginine deiminases (PADIs), converts arginine to citrulline, altering chromatin structure and regulating gene expression. This modification plays a role in epigenetic regulation, immune response, and disease pathogenesis, including cancer and autoimmune disorders like rheumatoid arthritis.
Histone citrullination is associated with chromatin decondensation, transcriptional activation, and neutrophil extracellular trap (NET) formation. The simultaneous detection of citrullination at R2. R8. and R17 highlights its importance in diverse biological contexts, such as embryonic development, inflammation, and cellular stress responses. This antibody is particularly valuable for studying autoimmune diseases, where aberrant citrullination of histones and other proteins generates autoantigens.
Researchers employ this antibody in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore histone modification dynamics and their functional implications. Its specificity for multiple citrullination sites makes it a versatile tool for dissecting the role of citrullinated histones in both physiological processes and pathological conditions.
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