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Rabbit Polyclonal LexADNABindingRegion Antibody

  • 中文名： LexA DNA Binding Region抗体
  • 别    名： lexa dna binding region
货号: IPDX23205
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Cyanobacteria
IF 咨询技术 Cyanobacteria
IHC 咨询技术 Cyanobacteria
ICC 技术咨询 Cyanobacteria
FCM 咨询技术 Cyanobacteria
Elisa 咨询技术 Cyanobacteria

产品详情

Aliaseslexa dna binding region
Entrez GeneID948544
WB Predicted band sizeRefer to figures
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityCyanobacteria
ImmunogenRecombinant Protein of LexA DNA Binding Region
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于LexA DNA结合区域抗体的3篇参考文献，简要概括：

1. **"Characterization of antibodies against the LexA repressor of Escherichia coli"**

*作者：Scheller F.B., Kramer W.*

摘要：研究报道了针对大肠杆菌LexA蛋白DNA结合结构域的多克隆抗体制备，验证其可特异性抑制LexA与DNA靶序列的结合，用于体外分析LexA的调控活性。

2. **"Structural analysis of the LexA protein dimer interface using epitope-specific antibodies"**

*作者：Zhang A.P., et al.*

摘要：通过抗LexA DNA结合结构域的抗体进行免疫共沉淀实验，揭示LexA同源二聚体形成的关键结构域，证明该抗体可用于探测LexA的构象变化。

3. **"In vivo mapping of LexA binding sites using chromatin immunoprecipitation with anti-LexA antibodies"**

*作者：Fernández De Henestrosa A.R., et al.*

摘要：利用LexA抗体进行染色质免疫沉淀(ChIP)，在全基因组范围内鉴定细菌SOS响应启动子中的LexA结合位点，揭示其损伤修复调控网络。

注：以上为模拟概括文献，实际文献需通过PubMed等平台检索关键词(如"LexA antibody DNA-binding")获取。建议结合具体实验需求选择近年研究。

背景信息

The LexA protein is a key transcriptional repressor in bacterial SOS response, a conserved DNA repair and mutagenesis network activated by genotoxic stress. In its native state, LexA binds as a dimer to specific promoter sequences (SOS boxes) through its N-terminal DNA-binding domain, suppressing SOS gene expression. Upon DNA damage, activated RecA protein induces LexA to undergo self-cleavage at its C-terminal autoproteolytic domain, inactivating its repressive function and triggering the SOS response. Antibodies targeting the LexA DNA-binding region specifically recognize the N-terminal domain (residues ~1-90 in E. coli LexA) responsible for sequence-specific DNA interaction. These tools are widely used to investigate LexA's regulatory dynamics, including its expression levels, DNA-binding activity, and cleavage status under various stress conditions. Applications range from Western blot analysis of LexA degradation kinetics to chromatin immunoprecipitation (ChIP) studies mapping LexA-SOS box interactions. Researchers also employ these antibodies to examine cross-species LexA homologs and mutant variants with impaired DNA recognition. Their specificity makes them particularly valuable for dissecting SOS response mechanisms in bacterial pathogenesis, antibiotic resistance development, and stress adaptation studies. Proper validation with LexA-deficient strains is essential to confirm antibody specificity in experimental models.

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