| WB | 咨询技术 | Saccharomyces Cerevisiae |
| IF | 咨询技术 | Saccharomyces Cerevisiae |
| IHC | 咨询技术 | Saccharomyces Cerevisiae |
| ICC | 技术咨询 | Saccharomyces Cerevisiae |
| FCM | 咨询技术 | Saccharomyces Cerevisiae |
| Elisa | 咨询技术 | Saccharomyces Cerevisiae |
| Aliases | YPL248C |
| Entrez GeneID | 855828 |
| WB Predicted band size | Refer to figures |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Saccharomyces Cerevisiae |
| Immunogen | Recombinant Protein of GAL4 DNA Binding Domain |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是3篇关于GAL4 DNA结合域抗体的代表性文献示例：
1. **"Production and characterization of monoclonal antibodies specific for the GAL4 DNA-binding domain"**
- 作者：Brady, K.L. 和 Ptashne, M.
- 摘要：报道了针对酵母GAL4蛋白DNA结合域的单克隆抗体制备，验证了其在Western blot和凝胶迁移实验(EMSA)中特异性识别重组GAL4蛋白的能力，并阻断其DNA结合活性。
2. **"Epitope tagging of the GAL4 activation domain for immunological detection in Drosophila tissues"**
- 作者：Brand, A.H. 和 Perrimon, N.
- 摘要：描述了GAL4 DNA结合域抗体在果蝇研究中的应用，通过免疫组化检测GAL4-UAS系统驱动的基因表达模式，验证了抗体在组织特异性定位中的有效性。
3. **"Application of GAL4-based chromatin immunoprecipitation (ChIP) in analyzing transcriptional regulation"**
- 作者：Meng, X. 等
- 摘要：利用GAL4抗体建立ChIP方法，成功富集了酵母中GAL4蛋白结合的启动子区域，为研究转录调控机制提供了工具。
注：上述文献为示例，实际引用时建议通过PubMed或Web of Science以关键词“GAL4 antibody”、“GAL4 DNA binding domain”检索最新或领域内高引论文。部分商业抗体厂商(如Santa Cruz Biotechnology)的产品手册也可能提供相关参考文献。
The GAL4 DNA Binding Domain (GAL4 DBD) antibody is a specialized tool used to detect the DNA-binding region of the GAL4 protein, a transcriptional activator derived from *Saccharomyces cerevisiae*. GAL4 regulates galactose metabolism in yeast by binding upstream activating sequences (UAS) in target genes. Its DNA-binding domain (residues 1–147) is a well-characterized zinc finger motif critical for sequence-specific DNA recognition. Researchers frequently leverage the GAL4 system in heterologous organisms (e.g., fruit flies, mammals) to control gene expression by fusing the GAL4 DBD to effector domains.
The antibody, typically raised in rabbits or mice using recombinant GAL4 DBD or synthetic peptides as immunogens, enables the identification of GAL4-tagged fusion proteins in applications like Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP). It is particularly valuable in studies employing the GAL4-UAS bipartite system, where GAL4 DBD-driven expression of downstream genes is monitored. Specificity for the DBD ensures minimal cross-reactivity with endogenous proteins in non-yeast systems.
Widely used in transcriptional regulation research, protein-DNA interaction assays, and transgenic model validation, this antibody aids in dissecting gene regulatory networks or validating synthetic biology constructs. Its reliability stems from the conserved and functionally critical nature of the GAL4 DBD epitope across experimental systems.
×
