| WB | 咨询技术 | Plant |
| IF | 咨询技术 | Plant |
| IHC | 咨询技术 | Plant |
| ICC | 技术咨询 | Plant |
| FCM | 咨询技术 | Plant |
| Elisa | 咨询技术 | Plant |
| Aliases | PGC; Gastricsin; Pepsinogen C |
| Entrez GeneID | 5225 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 105-109 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Plant |
| Immunogen | Synthetic Peptide of PEPC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PEPC(磷酸烯醇式丙酮酸羧化酶)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Production and Characterization of Monoclonal Antibodies Against Maize Phosphoenolpyruvate Carboxylase*
**作者**: Sánchez, R., et al.
**摘要**: 该研究报道了针对玉米PEPC的单克隆抗体制备，通过Western blot和免疫组织化学验证抗体特异性，成功用于玉米叶片中PEPC的亚细胞定位及C4植物光合途径的酶活性分析。
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2. **文献名称**: *Immunological Analysis of PEPC Isoforms in Arabidopsis thaliana*
**作者**: Kagaya, Y., et al.
**摘要**: 研究利用多克隆抗体检测拟南芥中PEPC的同工酶表达，揭示其在非光合组织(如根和种子)中的功能多样性，并证明抗体可特异性区分不同磷酸化修饰的PEPC形式。
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3. **文献名称**: *Development of a Cross-Species PEPC Antibody for Comparative Studies in C3 and C4 Plants*
**作者**: Chollet, R., et al.
**摘要**: 开发了一种广谱多克隆抗体，可识别多种植物(如水稻、高粱)的PEPC蛋白，通过比较C3和C4植物中酶的丰度与分布，验证抗体在进化及功能研究中的实用性。
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注：以上文献为示例，实际引用时需核实具体来源及发表信息。如需真实文献，建议通过PubMed或Web of Science检索关键词“PEPC antibody”或联系领域内综述获取。
**Background of PEPC Antibodies**
Phosphoenolpyruvate carboxylase (PEPC) is a key enzyme in plant metabolism, catalyzing the irreversible carboxylation of phosphoenolpyruvate (PEP) to oxaloacetate, a critical step in C4 and crassulacean acid metabolism (CAM) photosynthesis. It also plays roles in anaplerotic carbon flux, nitrogen assimilation, and stress responses. PEPC antibodies are immunological tools developed to detect and quantify this enzyme in various biological samples.
These antibodies are typically generated by immunizing hosts (e.g., rabbits, mice) with purified PEPC protein or synthetic peptides derived from conserved regions of the enzyme. Their specificity is validated through techniques like Western blotting, immunohistochemistry, or ELISA, often cross-reacting with PEPC isoforms across plant species due to sequence homology.
PEPC antibodies are widely used in plant physiology research to study enzyme expression, localization, and regulation under different conditions (e.g., light, nutrient availability, abiotic stress). They also aid in exploring metabolic engineering strategies to enhance crop productivity or stress tolerance. In agricultural biotechnology, these antibodies help monitor PEPC activity in genetically modified organisms. Challenges include distinguishing between photosynthetic and non-photosynthetic PEPC isoforms, necessitating careful experimental design. Overall, PEPC antibodies remain indispensable for dissecting carbon fixation mechanisms and improving sustainable agriculture.
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