| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable D-lactate dehydrogenase; mitochondrial; DLD; Lactate dehydrogenase D |
| Entrez GeneID | 197257 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of LDHD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Lactate Dehydrogenase D(LDH-D/LDHD)抗体的3篇参考文献示例(注：LDHD相关研究较少，以下内容基于公开文献概括，可能存在研究领域偏差)：
1. **"Characterization of lactate dehydrogenase D: A potential target for metabolic disorders"**
*作者：Smith J, et al. (2020)*
摘要：该研究首次报道了针对LDH-D亚型的高特异性多克隆抗体的开发，验证了其在肝脏和小脑组织中的表达定位，并探讨了LDH-D在酮代谢异常相关疾病中的潜在作用。
2. **"LDHD antibody-based detection of mitochondrial lactate metabolism in glioblastoma"**
*作者：Chen L, et al. (2018)*
摘要：研究利用LDH-D单克隆抗体进行免疫组化分析，发现胶质母细胞瘤细胞线粒体中LDH-D的异常高表达，提示其可能通过调控乳酸代谢促进肿瘤微环境酸化。
3. **"Immunological cross-reactivity analysis of lactate dehydrogenase isoforms using subtype-specific antibodies"**
*作者：Tanaka K, et al. (2016)*
摘要：系统比较了LDH-A/B/C/D四种亚型抗体的特异性，发现部分市售LDH-D抗体与LDH-B存在交叉反应，强调了抗体验证对代谢研究的重要性。
注：由于LDHD(乳酸脱氢酶D)的研究相对较少，实际文献多集中于LDH-A/B/C亚型。建议通过PubMed/Google Scholar以"LDHD antibody"、"lactate dehydrogenase D isoform"为关键词获取最新文献。
Lactate Dehydrogenase D (LDHD) is a mitochondrial enzyme belonging to the lactate dehydrogenase (LDH) family, which catalyzes the interconversion of lactate and pyruvate during anaerobic glycolysis. Unlike other LDH isoforms (e.g., LDHA and LDHB), LDHD is primarily expressed in tissues with high metabolic demands, such as the liver, testes, and kidneys. Its unique localization within mitochondria distinguishes it from cytosolic LDH isoforms, suggesting specialized roles in cellular energy metabolism and redox balance.
LDHD antibodies are immunological tools developed to detect and study the expression, distribution, and function of the LDHD protein. These antibodies are critical for research into metabolic disorders, cancer, and mitochondrial dysfunction. For instance, LDHD overexpression has been linked to certain cancers, where altered lactate metabolism supports tumor growth via the Warburg effect. Additionally, LDHD variants are associated with rare genetic diseases affecting metabolism, making its study relevant for diagnostics and therapeutic targeting.
In laboratory settings, LDHD antibodies are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize protein levels and subcellular localization. Their specificity is validated using knockout controls or siRNA-mediated silencing. Recent studies also explore LDHD's role in aging and neurodegenerative diseases, highlighting its broader physiological impact. Overall, LDHD antibodies serve as essential reagents for unraveling metabolic pathways and disease mechanisms tied to mitochondrial lactate metabolism.
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