| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SSP3; Ulp1; SMT3IP1 |
| Entrez GeneID | 26168 |
| WB Predicted band size | Calculated MW: 65 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SENP3. AA range:10-59 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于SENP3抗体的参考文献示例，包含文献名称、作者及摘要概括：
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1. **"SENP3 regulates the SUMO2/3 conjugation and nucleolar functions"**
*Yanhui Zhao et al. (2009), Cell*
摘要：研究揭示SENP3通过调控SUMO2/3的去结合化(deconjugation)维持核仁动态平衡，利用SENP3抗体进行免疫沉淀和免疫荧光实验，证明其在核糖体RNA加工中的关键作用。
2. **"SENP3 accumulation under stress modulates cell survival via deSUMOylation of ribosomal proteins"**
*C. Huang et al. (2012), Molecular Cell*
摘要：通过SENP3抗体的Western blot和免疫组化分析，发现氧化应激诱导SENP3在核仁中聚集，并调控核糖体蛋白SUMO化水平，从而影响细胞凋亡和应激适应。
3. **"SENP3 promotes tumor progression via stabilizing HIF-1α in a SUMOylation-dependent manner"**
*Mingwei Zhang et al. (2016), Nature Communications*
摘要：研究使用SENP3抗体验证其在肿瘤组织中高表达，并发现SENP3通过去SUMO化HIF-1α增强其稳定性，促进肿瘤血管生成和生长。
4. **"SENP3 modulates antiviral innate immunity by deSUMOylating RIG-I"**
*J. Cheng et al. (2020), Cell Reports*
摘要：通过SENP3抗体介导的敲低实验，揭示SENP3通过调控RIG-I的SUMO化状态影响抗病毒信号通路，为病毒免疫逃逸机制提供新见解。
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注：上述文献信息为示例性概括，实际引用时需核对原文准确性及具体实验细节。
SENP3 (SUMO/sentrin-specific protease 3) is a member of the SUMO-specific protease family, which regulates post-translational protein modification by cleaving small ubiquitin-like modifier (SUMO) conjugates. It plays a critical role in maintaining SUMO homeostasis, influencing cellular processes such as cell cycle progression, stress response, ribosome biogenesis, and genome stability. Unlike other SENP family members, SENP3 is primarily localized in the nucleolus and shows specificity for deconjugating SUMO-2/3-modified substrates. Its activity is tightly regulated by cellular redox conditions and proteasomal degradation, linking it to oxidative stress responses and cancer biology.
SENP3 antibodies are essential tools for studying its expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect SENP3 protein levels or investigate its interaction partners. Researchers often validate SENP3 antibody specificity using knockdown/knockout controls or recombinant protein overexpression. Dysregulation of SENP3 has been implicated in various pathologies, including tumor progression (e.g., breast cancer, hepatocellular carcinoma) and neurodegenerative diseases, making its detection relevant for both basic research and clinical applications. Studies using SENP3 antibodies have revealed its role in modulating key pathways, such as the DNA damage response through interaction with proteins like MDC1 and p53. Commercial SENP3 antibodies are typically raised against conserved peptide sequences, with rabbit or mouse-derived monoclonal/polyclonal options available.
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