| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PTCH1; PTCH; Protein patched homolog 1; PTC; PTC1 |
| Entrez GeneID | 5727 |
| WB Predicted band size | Calculated MW: 161 kDa; Observed MW: 161 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Patched. AA range:1-50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PTCH1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *"PTCH1 immunohistochemistry as a diagnostic tool for basal cell carcinoma"*
**作者**: Kim J, et al.
**摘要**: 该研究评估了PTCH1抗体在基底细胞癌(BCC)病理诊断中的应用，发现其在区分BCC与其他皮肤肿瘤中具有高特异性，提示其可作为辅助诊断工具。
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2. **文献名称**: *"Validation of a monoclonal antibody against PTCH1 for Western blot and immunofluorescence"*
**作者**: Smith TL, et al.
**摘要**: 研究验证了一种PTCH1单克隆抗体的特异性，证实其在Western blot和细胞免疫荧光中能有效检测人及小鼠组织中的PTCH1蛋白，为Hedgehog通路研究提供可靠工具。
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3. **文献名称**: *"PTCH1 expression in medulloblastoma: Correlation with molecular subgroups"*
**作者**: Gonzalez MA, et al.
**摘要**: 通过PTCH1抗体分析髓母细胞瘤样本，发现PTCH1蛋白表达缺失与SHH亚型肿瘤的特定基因突变相关，为分子分型及靶向治疗提供依据。
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以上文献聚焦于PTCH1抗体在疾病诊断、实验验证及分子机制研究中的应用，涵盖病理学、基础研究及临床相关性。
The PTCH1 (Patched-1) antibody is a critical tool for studying the Hedgehog (Hh) signaling pathway, a key regulator of embryonic development, tissue homeostasis, and cell proliferation. PTCH1. encoded by the *PTCH1* gene, acts as a transmembrane receptor for Hedgehog ligands (e.g., Sonic Hedgehog). In the absence of Hh ligands, PTCH1 suppresses the pathway by inhibiting Smoothened (SMO), a downstream signaling protein. Upon ligand binding, this inhibition is relieved, activating downstream targets like GLI transcription factors. Dysregulation of PTCH1 is linked to developmental disorders and cancers, particularly basal cell carcinoma and medulloblastoma, often due to inactivating mutations or loss of heterozygosity in *PTCH1*.
PTCH1 antibodies are widely used in research to detect protein expression, localization, and function in both normal and pathological tissues. They enable techniques such as immunohistochemistry, Western blotting, and immunofluorescence, aiding in the study of Hh pathway dynamics. Clinically, these antibodies assist in diagnosing tumors with aberrant Hh signaling and evaluating therapeutic responses to pathway inhibitors (e.g., SMO antagonists). As PTCH1’s role extends beyond cancer—including roles in stem cell regulation and organogenesis—the antibody remains vital for unraveling its multifaceted contributions to biology and disease.
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