| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS6KA5; MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL |
| Entrez GeneID | 9252 |
| WB Predicted band size | Calculated MW: 90 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human MSK1 around the non-phosphorylation site of T581. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MSK1抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: "MSK1 regulates NF-κB signaling through phosphorylation of histone H3 in inflammatory responses"
**作者**: Smith A, et al.
**摘要**: 研究利用MSK1特异性抗体，揭示MSK1通过磷酸化组蛋白H3(Ser10)调控NF-κB介导的炎症基因表达，在巨噬细胞模型中验证其对抗原刺激的响应机制。
2. **文献名称**: "Role of MSK1 in CREB activation and neuronal survival under oxidative stress"
**作者**: Lee JH, et al.
**摘要**: 通过Western blot和免疫荧光技术(使用MSK1抗体)，证明MSK1在神经元氧化应激中磷酸化CREB蛋白，促进抗凋亡基因表达，维持细胞存活。
3. **文献名称**: "MSK1 interacts with p38 MAPK to mediate UV-induced DNA damage repair"
**作者**: Chen X, et al.
**摘要**: 利用MSK1抗体进行免疫共沉淀实验，发现MSK1与p38 MAPK形成复合物，调控紫外线诱导的DNA损伤修复通路，影响细胞周期停滞与修复效率。
4. **文献名称**: "Tissue-specific expression of MSK1 revealed by antibody-based profiling in mouse models"
**作者**: Rodriguez B, et al.
**摘要**: 通过多种MSK1抗体的免疫组化分析，系统描绘了MSK1在小鼠脑、肝脏及免疫组织中的差异表达，提示其功能在器官发育和疾病中的潜在特异性。
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**注**：以上文献为虚构示例，实际研究中建议通过PubMed或Google Scholar检索关键词(如"MSK1 antibody"、"MSK1 kinase function")获取真实文献。
The MSK1 (Mitogen- and Stress-Activated Protein Kinase 1) antibody is a research tool designed to detect and study the MSK1 protein, a serine/threonine kinase involved in intracellular signaling pathways. MSK1. encoded by the *RPS6KA5* gene, plays a critical role in mediating cellular responses to mitogens, stress stimuli, and extracellular signals such as growth factors or inflammatory cytokines. It is activated downstream of the ERK (Extracellular Signal-Regulated Kinase) and p38 MAPK (Mitogen-Activated Protein Kinase) pathways, which phosphorylate MSK1 to regulate its enzymatic activity. Once activated, MSK1 translocates to the nucleus, where it phosphorylates transcription factors like CREB (cAMP Response Element-Binding Protein) and histone H3. linking extracellular signals to chromatin remodeling and gene expression.
MSK1 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate MSK1 expression, localization, and activation status (e.g., phosphorylation at specific residues like Ser376). These antibodies help researchers explore MSK1's roles in processes such as inflammation, cell survival, and differentiation, as well as its implications in diseases like cancer, neurodegenerative disorders, and immune dysregulation. Validation of MSK1 antibodies is critical, as cross-reactivity with homologous kinases (e.g., MSK2) may occur. Overall, MSK1 antibodies serve as essential tools for dissecting kinase-driven signaling networks and their physiological or pathological outcomes.
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