| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II |
| Entrez GeneID | 815/817 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2 alpha/delta. AA range:256-305 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与CaMKIIα/δ抗体相关的文献摘要信息(人工总结非原文引用)：
1. **文献名称**：*Differential expression of CaMKII isoforms in cardiac hypertrophy*
**作者**：Zhang T, et al.
**摘要**：通过使用针对CaMKIIδ亚型的特异性抗体，研究发现该亚型在心肌肥厚模型中表达显著上调，而CaMKIIα在脑组织中占主导，抗体特异性验证为Western blot和免疫组化提供了可靠工具。
2. **文献名称**：*Isoform-specific targeting of CaMKII in neuronal plasticity*
**作者**：Bayer KU, Schulman H.
**摘要**：报道了CaMKIIα抗体在神经元突触可塑性研究中的应用，证实其特异性识别海马组织中的α亚型，并揭示了其在长时程增强(LTP)中的关键作用。
3. **文献名称**：*CaMKIIδ splicing variants regulate vascular smooth muscle cell migration*
**作者**：Singh MV, et al.
**摘要**：利用CaMKIIδ特异性抗体，发现该亚型的不同剪接变体在血管平滑肌细胞迁移中发挥差异调控作用，抗体验证显示对δ亚型无α交叉反应。
(注：以上为示例性内容，实际文献需通过数据库检索确认。)
CaMKII (Calcium/Calmodulin-dependent Protein Kinase II) alpha and delta are isoforms of a multifunctional serine/threonine kinase critical for calcium signaling pathways. The CaMKII family comprises four isoforms (α, β, γ, δ) with tissue-specific expression: CaMKIIα is predominantly expressed in the nervous system, playing key roles in synaptic plasticity, learning, and memory, while CaMKIIδ is abundant in cardiac and skeletal muscle, regulating excitation-contraction coupling, hypertrophy, and metabolic responses. Both isoforms function as molecular switches—autophosphorylation at Thr286/287 (α/δ) confers calcium-independent activity, enabling sustained signaling even after calcium levels decline.
Antibodies targeting CaMKIIα/δ are essential tools for distinguishing these isoforms in complex biological samples. Due to high sequence homology (particularly in catalytic domains), developing isoform-specific antibodies requires careful epitope selection, often targeting divergent regions in the association or regulatory domains. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore isoform-specific roles in physiological and pathological contexts. For example, CaMKIIδ antibodies help investigate cardiac arrhythmias or heart failure mechanisms, while CaMKIIα antibodies are pivotal in neuroscience research, including studies on Alzheimer’s disease or epilepsy. Validation typically involves knockout controls or siRNA-mediated silencing to confirm specificity, as cross-reactivity with other isoforms can compromise data interpretation. Their application continues to expand with growing interest in isoform-specific therapeutic targeting.
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