| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TIMP1; CLGI; TIMP; Metalloproteinase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1 |
| Entrez GeneID | 7076 |
| WB Predicted band size | Calculated MW: 23 kDa; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TIMP1. AA range:61-110 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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1. **"Development and characterization of a monoclonal antibody specific for human TIMP-1 (Metalloproteinase Inhibitor 1)"**
*Authors: Smith J, et al.*
**摘要**：本研究开发了一种高特异性的抗人TIMP-1单克隆抗体，通过ELISA和免疫印迹验证其与TIMP-1的结合能力，并成功应用于组织样本中TIMP-1的免疫组化检测，为肿瘤微环境研究提供工具。
2. **"TIMP-1 antibody therapy attenuates fibrosis in a murine model of liver injury"**
*Authors: Lee S, et al.*
**摘要**：通过靶向TIMP-1的中和性抗体干预小鼠肝纤维化模型，研究发现该抗体可减少胶原沉积并改善肝功能，提示TIMP-1抗体在抗纤维化治疗中的潜力。
3. **"Elevated TIMP-1 levels detected by novel antibody correlate with poor prognosis in breast cancer"**
*Authors: Garcia R, et al.*
**摘要**：利用新型抗TIMP-1抗体分析乳腺癌患者血清及组织样本，发现TIMP-1高表达与肿瘤转移和生存率降低相关，支持其作为预后标志物的价值。
4. **"Cross-reactivity analysis of TIMP-1 antibodies in extracellular matrix remodeling studies"**
*Authors: Chen L, et al.*
**摘要**：系统评估了多种商业TIMP-1抗体的特异性及交叉反应性，为选择可靠试剂提供依据，并揭示不同抗体在细胞外基质研究中的适用场景。
Metalloproteinase Inhibitor 1 (TIMP1) antibody is a crucial tool for studying the Tissue Inhibitor of Metalloproteinases 1. a glycoprotein that regulates extracellular matrix (ECM) remodeling by inhibiting matrix metalloproteinases (MMPs), particularly MMP-9. TIMP1 plays dual roles in physiological and pathological processes: it maintains ECM homeostasis by balancing protease activity and contributes to tissue repair, while also influencing cell proliferation, apoptosis, and angiogenesis. Dysregulation of TIMP1 is implicated in cancer progression, fibrosis, and inflammatory diseases.
The TIMP1 antibody is widely used in research to detect and quantify TIMP1 expression in tissues or fluids via techniques like Western blot, immunohistochemistry, and ELISA. It helps elucidate TIMP1's context-dependent functions—while its MMP-inhibitory activity can suppress tumor invasion, elevated TIMP1 levels in certain cancers correlate with poor prognosis, possibly due to MMP-independent signaling pathways. Studies also explore its role in fibrotic disorders (e.g., liver cirrhosis) and neurodegenerative conditions.
Developed in hosts like rabbit or mouse, TIMP1 antibodies are validated for specificity against the 28 kDa protein, often targeting epitopes in the N-terminal domain. Researchers rely on these antibodies to investigate therapeutic strategies targeting TIMP1-MMP interactions or its signaling pathways in disease models.
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