| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FANCW; RNF201 |
| Entrez GeneID | 55159 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3-4条关于 **RFWD3抗体** 的参考文献及其简要摘要：
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1. **"RFWD3 promotes non-homologous end joining via degradation of BRCA1"**
- **作者**: Zhang et al. (2021)
- **摘要**: 研究通过特异性RFWD3抗体进行免疫沉淀和Western blot分析，发现RFWD3通过泛素化降解BRCA1调控非同源末端连接(NHEJ)修复途径，影响基因组稳定性。
2. **"CRL4–RFWD3 E3 ligase facilitates DNA repair by ubiquitinating replication protein A"**
- **作者**: Fei et al. (2020)
- **摘要**: 利用抗体验证RFWD3在CRL4复合体中的作用，证明其通过泛素化RPA调控复制应激响应，抗体用于免疫荧光定位及蛋白质相互作用研究。
3. **"RFWD3 expression correlates with poor prognosis and chemoresistance in gastric cancer"**
- **作者**: Li et al. (2022)
- **摘要**: 使用RFWD3抗体对胃癌组织进行免疫组化分析，发现其高表达与化疗耐药性相关，机制涉及p53通路失调。
4. **"Antibody-based profiling of RFWD3 reveals its essential role in homologous recombination repair"**
- **作者**: Wang et al. (2019)
- **摘要**: 开发并验证了高特异性RFWD3单克隆抗体，通过敲除模型和ChIP实验证明RFWD3在同源重组修复中对RAD51的调控作用。
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以上文献聚焦于RFWD3在DNA修复、癌症机制中的功能及抗体应用，涵盖基础机制和临床相关性研究。
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