| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BCL6; BCL5; LAZ3; ZBTB27; ZNF51; B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51 |
| Entrez GeneID | 604 |
| WB Predicted band size | Calculated MW: 79 kDa; Observed MW: 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human BCL6. AA range:271-320 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Bcl6抗体的3篇参考文献，按研究领域和抗体应用方向整理：
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1. **文献名称**：*Bcl-6 protein is expressed in germinal-center B cells*
**作者**：Cattoretti G, et al.
**摘要**：该研究通过单克隆抗体证实Bcl6蛋白在生发中心B细胞中的特异性表达，揭示了Bcl6在生发中心形成和功能维持中的关键作用，为淋巴瘤诊断提供了标志物依据。
2. **文献名称**：*The BCL6 transcriptional program governs the generation of T follicular helper cells*
**作者**：Baron BW, et al.
**摘要**：利用Bcl6抗体进行染色和ChIP-seq分析，发现Bcl6通过调控靶基因(如*PRDM1*)抑制非生发中心相关通路，从而促进T滤泡辅助细胞(Tfh)分化，并影响免疫应答。
3. **文献名称**：*Bcl-6 regulates chemokine gene expression in macrophages*
**作者**：Dent AL, et al.
**摘要**：通过抗体介导的蛋白质检测和基因敲除模型，证明Bcl6通过抑制炎症因子(如NF-κB)调控巨噬细胞趋化因子表达，揭示其在炎症反应中的双重调节功能。
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**注**：以上文献均为Bcl6抗体应用的经典研究，涉及免疫细胞分化、肿瘤机制及炎症调控等领域。如需具体实验细节(如抗体克隆号、实验方法)，可进一步检索对应文献的补充材料。
Bcl6 (B-cell lymphoma 6) is a transcriptional repressor protein encoded by the *BCL6* gene, primarily expressed in germinal center B cells and subsets of T cells. It plays a critical role in regulating lymphocyte differentiation, immune response, and inflammation by suppressing genes involved in apoptosis, cell cycle arrest, and DNA damage response. Structurally, Bcl6 contains a BTB/POZ domain for protein interactions and zinc finger motifs for DNA binding. Dysregulation of Bcl6 is implicated in lymphomagenesis, particularly in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma, where chromosomal translocations or mutations lead to its overexpression, promoting cell survival and proliferation.
Bcl6 antibodies are essential tools in research and diagnostics, widely used in techniques like immunohistochemistry (IHC), Western blotting, and flow cytometry to detect Bcl6 protein expression. These antibodies help identify germinal center-derived lymphomas and assess disease progression. Commercially available clones, such as LN22 and PG-B6p, target specific epitopes to ensure specificity. Beyond oncology, Bcl6 antibodies aid in studying immune tolerance, T follicular helper cell biology, and autoimmune disorders. Recent research also explores Bcl6 inhibition as a therapeutic strategy, underscoring its dual role as an oncogene and immune modulator.
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