| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAX5; Paired box protein Pax-5; B-cell-specific transcription factor; BSAP |
| Entrez GeneID | 5079 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around aa.6~10(N-Y-P-T-P) derived from Human Pax5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于PAX5抗体的参考文献及其简要摘要：
1. **"PAX5 immunohistochemistry in the diagnosis of B-cell lymphomas"**
- **作者**: Töröcsik D. 等
- **摘要**: 该研究验证了PAX5抗体在区分B细胞淋巴瘤(如弥漫大B细胞淋巴瘤)与其他淋巴组织增生性疾病中的特异性，强调其作为B细胞标志物的临床诊断价值。
2. **"PAX5 expression in acute leukemias: lineage-specificity and genetic alterations"**
- **作者**: Shapiro L.H., Cheng G.
- **摘要**: 通过免疫组化和分子分析，揭示了PAX5在B系急性白血病(B-ALL)中的特异性表达，并探讨了PAX5基因突变与白血病发生的关系。
3. **"The role of PAX5 in B-cell differentiation and its aberrant expression in cancers"**
- **作者**: Dörfler P., Busslinger M.
- **摘要**: 综述PAX5在正常B细胞发育中的转录调控功能，并讨论其异常表达或突变如何导致B细胞恶性肿瘤(如淋巴瘤和白血病)的发生机制。
The PAX5 antibody is a crucial tool in immunology and diagnostic pathology, targeting the PAX5 protein encoded by the *PAX5* gene on chromosome 9p13. PAX5. a member of the paired box (PAX) transcription factor family, is essential for B-cell lineage commitment, differentiation, and maintenance. It regulates genes involved in B-cell receptor signaling (e.g., *CD19*, *BLNK*) and suppresses alternative hematopoietic lineages, ensuring B-cell identity. PAX5 expression is largely restricted to B-cells at various maturation stages, from pro-B cells to mature B-lymphocytes, but is absent in plasma cells and non-B-cell lineages.
In diagnostics, PAX5 antibodies are widely used in immunohistochemistry (IHC) and flow cytometry to identify B-cell malignancies, such as B-cell lymphomas and B-cell acute lymphoblastic leukemia (B-ALL). Its nuclear staining pattern helps distinguish B-cell neoplasms from T-cell or myeloid malignancies. PAX5 is also a marker for classic Hodgkin lymphoma, where Reed-Sternberg cells often exhibit weak or variable PAX5 expression. Additionally, PAX5 loss or mutations are implicated in B-cell development disorders and certain cancers, underscoring its role in tumorigenesis.
Research applications include studying B-cell development, differentiation, and PAX5’s interplay with oncogenic pathways. However, cross-reactivity with other PAX family proteins requires careful validation. Overall, PAX5 antibodies remain indispensable for both clinical diagnostics and molecular research in hematopathology.
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