| WB | 1/500-1/1000 | Streptococcus pyogenes |
| IF | 咨询技术 | Streptococcus pyogenes |
| IHC | 1/50-1/100 | Streptococcus pyogenes |
| ICC | 1/50-1/200 | Streptococcus pyogenes |
| FCM | 1/50-1/100 | Streptococcus pyogenes |
| Elisa | 咨询技术 | Streptococcus pyogenes |
| Aliases | Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9 |
| Entrez GeneID | - |
| WB Predicted band size | Calculated MW: 158 kDa; Observed MW: 158 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Streptococcus pyogenes |
| Immunogen | A synthesized peptide derived from CRISPR-Cas9 SP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CRISPR-Cas9 SP抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*CRISPR-Cas9 knockin mice for genome editing and cancer modeling*
**作者**：Platt et al. (2014)
**摘要**：本研究利用SpCas9转基因小鼠模型，通过Western blot和免疫组化实验验证Cas9蛋白的表达，使用特异性SpCas9抗体检测其在多种组织中的分布。结果显示Cas9在体内广泛表达，为后续基因编辑研究提供工具。
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2. **文献名称**：*Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells*
**作者**：Shalem et al. (2014)
**摘要**：作者开发了全基因组CRISPR筛选平台，利用SpCas9抗体进行免疫印迹分析，确认Cas9在细胞中的稳定表达。研究验证了抗体在检测CRISPR系统效率中的关键作用，并鉴定出多个癌症相关基因。
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3. **文献名称**：*Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA*
**作者**：Nishimasu et al. (2014)
**摘要**：该研究解析了SpCas9蛋白与RNA及DNA复合物的晶体结构，通过抗SpCas9抗体进行免疫沉淀实验，验证蛋白复合物的纯度与构象，为Cas9的分子机制提供结构基础。
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**说明**：以上文献中使用的SpCas9抗体多用于Western blot、免疫组化或免疫沉淀，以验证Cas9表达或分析其功能。实际应用中，可参考抗体供应商(如Abcam、Cell Signaling Technology)提供的产品引用文献获取更具体信息。
CRISPR-Cas9 is a revolutionary genome-editing tool derived from bacterial immune systems, where the Cas9 endonuclease precisely targets and cuts DNA guided by a single-guide RNA (sgRNA). To study and optimize this system, researchers require reliable tools to detect and validate Cas9 expression and function. The CRISPR Cas9 SP antibody is a monoclonal or polyclonal antibody specifically developed to recognize the Cas9 protein, often targeting conserved epitopes within its C-terminal or N-terminal domains. These antibodies are critical for applications like Western blotting, immunofluorescence, and immunoprecipitation, enabling the visualization of Cas9 localization, expression levels, and interactions in cells or experimental models.
The development of Cas9-specific antibodies addressed challenges in verifying CRISPR experiments, ensuring that gene-editing outcomes correlate with proper Cas9 activity. High-quality SP antibodies exhibit minimal cross-reactivity with other cellular proteins, ensuring specificity. They are widely used in functional studies, quality control of Cas9-expressing cell lines, and troubleshooting experimental conditions. As CRISPR technology expands into therapeutics and biotechnology, Cas9 antibodies also play roles in safety assessments, such as detecting off-target effects or immune responses. Their utility underscores the importance of robust molecular tools in advancing CRISPR-based research and applications.
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