| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1 |
| Entrez GeneID | 6935 |
| WB Predicted band size | Calculated MW: 124 kDa; Observed MW: 200 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human AREB6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇关于ZEB1抗体的参考文献概览(均为虚拟示例，实际文献需通过学术数据库检索)：
---
1. **文献名称**：*ZEB1 Antibody Validation in Epithelial-Mesenchymal Transition Models*
**作者**：Smith A, et al.
**摘要**：本研究系统验证了多种商业ZEB1抗体的特异性，通过siRNA敲低和过表达实验，确认了抗体在Western blot和免疫荧光中的可靠性，并揭示了ZEB1在乳腺癌细胞EMT中的动态表达变化。
---
2. **文献名称**：*ZEB1 Immunohistochemical Staining Correlates with Metastasis in Colorectal Cancer*
**作者**：Zhang L, et al.
**摘要**：利用ZEB1抗体对200例结直肠癌组织进行免疫组化分析，发现高ZEB1表达与淋巴结转移和患者预后不良显著相关，提示其作为生物标志物的潜力。
---
3. **文献名称**：*Comparative Study of ZEB1 Antibodies in ChIP-seq Applications*
**作者**：Brown K, et al.
**摘要**：对比了三种ZEB1抗体在染色质免疫沉淀(ChIP-seq)中的性能，鉴定出抗体A在识别ZEB1与靶基因启动子结合时的最高特异性，为表观遗传研究提供工具选择依据。
---
如需真实文献，建议在PubMed或Google Scholar中搜索关键词："ZEB1 antibody validation" 或 "ZEB1 immunohistochemistry"。
The ZEB1 (Zinc Finger E-Box Binding Homeobox 1) antibody is a key tool for studying the ZEB1 protein, a transcription factor central to epithelial-mesenchymal transition (EMT) and cancer progression. ZEB1 binds E-box DNA motifs, repressing epithelial genes (e.g., E-cadherin) while activating mesenchymal genes, driving cell plasticity, metastasis, and therapeutic resistance in cancers. Its role extends beyond EMT, influencing stemness, apoptosis, and immune evasion. Aberrant ZEB1 expression correlates with poor prognosis in multiple cancers, including breast, lung, and colorectal.
ZEB1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression and localization in tissues or cell lines. Most commercial antibodies are raised against epitopes in the N- or C-terminal regions. Validation often includes knockout cell controls, tissue specificity checks, and cross-reactivity assessments with ZEB2. a closely related family member. Researchers prioritize antibodies validated for specific applications, as performance may vary. Recent studies also employ ZEB1 antibodies in ChIP-seq to map DNA binding sites or in co-immunoprecipitation to identify interaction partners. However, challenges persist due to ZEB1's low abundance in some contexts and nuclear localization, requiring optimized protocols for accurate detection.
×
