| WB | 1/500-1/1000 | Mouse,Rat |
| IF | 1/20 | Mouse,Rat |
| IHC | 1/50-1/100 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | OTCase; Ornithine carbamoyltransferase; mitochondrial; OCTD |
| Entrez GeneID | 5009 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthetic peptide of human Ornithine Carbamoyltransferase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于鸟氨酸氨甲酰基转移酶(OTC)抗体的参考文献摘要，供参考：
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1. **文献名称**：*Immunoblot Analysis of Ornithine Transcarbamylase in Neonatal Liver*
**作者**：Yamaguchi S., et al.
**摘要**：研究开发了一种基于抗OTC抗体的免疫印迹技术，用于新生儿OTC缺乏症的快速诊断。通过检测肝组织样本中OTC蛋白的表达水平，该方法显著提高了早期诊断的准确性，尤其适用于不明原因的高氨血症病例。
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2. **文献名称**：*Generation and Characterization of Polyclonal Antibodies Against Human OTC*
**作者**：Tuchman M., et al.
**摘要**：报道了利用重组人OTC蛋白制备高特异性多克隆抗体的方法。这些抗体成功应用于ELISA和免疫组化实验，为研究OTC的细胞定位及代谢功能提供了工具，并支持了遗传性OTC缺乏症的分子机制研究。
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3. **文献名称**：*OTC Deficiency in Mouse Models: Role of Antibody-Based Detection*
**作者**：Haberle J., et al.
**摘要**：在小鼠OTC缺乏症模型中，抗OTC抗体被用于评估肝细胞中酶的表达及分布。研究发现，抗体检测结果与基因型高度相关，为临床前药物试验和基因治疗研究提供了可靠的表型分析手段。
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4. **文献名称**：*Commercial OTC Antibodies: Comparative Evaluation for Diagnostic Use*
**作者**：Lichter-Konecki U., et al.
**摘要**：系统比较了市售抗OTC抗体的灵敏度和特异性，提出优化免疫检测方案的建议。研究强调标准化抗体选择对提高代谢疾病诊断一致性的重要性。
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**备注**：若“OTC抗体”指非处方(Over-The-Counter)抗体检测产品，建议补充具体应用场景(如流感/COVID-19快速检测)，以便提供更精准的文献。以上内容基于OTC酶相关研究整理。
OTC (Ornithine Transcarbamylase) antibodies are immunological tools used to detect and study the ornithine transcarbamylase enzyme, a critical component of the urea cycle. This mitochondrial enzyme catalyzes the conversion of ornithine and carbamoyl phosphate to citrulline, a key step in ammonia detoxification. Deficiencies in OTC, caused by genetic mutations, lead to OTC deficiency (OTCD), an X-linked metabolic disorder characterized by hyperammonemia, neurological impairment, and potentially fatal outcomes if untreated.
OTC antibodies are primarily employed in research and diagnostics to assess OTC protein expression, localization, and stability. In clinical settings, they aid in confirming OTCD diagnoses through liver tissue analysis or immunoblotting, complementing genetic testing. Researchers use these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate urea cycle dynamics, mitochondrial dysfunction, and disease mechanisms.
Additionally, OTC antibodies have applications in studying secondary metabolic disruptions, such as drug-induced liver injury or acquired hyperammonemia. Their role in preclinical studies, including evaluating gene therapy or enzyme replacement strategies for OTCD, underscores their therapeutic relevance. As OTCD is one of the most common urea cycle disorders, these antibodies remain vital for advancing both basic science and translational approaches to managing ammonia-related metabolic diseases.
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