| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I |
| Entrez GeneID | 5725 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PTBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇与PTBP1抗体相关的研究文献(信息基于公开研究总结，具体细节请参考原文)：
1. **"PTBP1 controls the reprogramming of human somatic cells into pluripotent stem cells"**
- **作者**: Xue Y. et al. (2013)
- **摘要**: 研究通过Western blot和免疫荧光技术使用PTBP1抗体，揭示了PTBP1在体细胞重编程为多能干细胞中的抑制作用，证明其调控关键剪接因子表达。
2. **"The RNA-binding protein PTBP1 promotes the expansion of adult hematopoietic stem cells"**
- **作者**: Wang J. et al. (2015)
- **摘要**: 利用PTBP1抗体进行免疫共沉淀和流式细胞分析，发现PTBP1通过调控RNA稳定性促进造血干细胞的增殖和维持。
3. **"PTBP1 knockdown induces apoptosis in glioblastoma cells through aberrant splicing of MDM4"**
- **作者**: Park Y. et al. (2017)
- **摘要**: 通过siRNA敲低PTBP1并结合PTBP1抗体的免疫印迹验证，研究揭示了PTBP1在胶质母细胞瘤中调控凋亡相关基因剪接的机制。
4. **"PTBP1 mediates the regulation of virus-triggered signaling by antagonizing TRAF3"**
- **作者**: Liu B. et al. (2019)
- **摘要**: 研究使用PTBP1抗体进行免疫沉淀和蛋白质互作分析，提出PTBP1通过抑制TRAF3增强抗病毒天然免疫反应的分子通路。
建议通过PubMed或Google Scholar检索具体文献，获取全文及实验细节。
**Background of PTBP1 Antibody**
PTBP1 (Polypyrimidine Tract-Binding Protein 1), also known as hnRNP I, is an RNA-binding protein involved in post-transcriptional gene regulation. It belongs to the heterogeneous nuclear ribonucleoprotein (hnRNP) family and plays critical roles in pre-mRNA splicing, mRNA stability, and translation. PTBP1 binds to pyrimidine-rich sequences in target RNAs through its RNA recognition motifs (RRMs), influencing splice site selection and isoform expression. Its activity is particularly significant in cellular processes like differentiation, proliferation, and apoptosis.
PTBP1 antibodies are essential tools for studying its expression, localization, and function. In research, these antibodies are used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect PTBP1 in various tissues and cell types. Dysregulation of PTBP1 is linked to cancers (e.g., glioblastoma, colorectal cancer) and neurological disorders, where its overexpression or altered splicing activity contributes to disease progression. For example, PTBP1 is implicated in repressing neuronal differentiation by regulating alternative splicing of transcripts critical for cell fate. Antibodies against PTBP1 also aid in exploring its role in viral infections, as some pathogens exploit PTBP1 to manipulate host RNA processing. Overall, PTBP1 antibodies are vital for unraveling its complex regulatory networks and potential therapeutic targets.
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