| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLN80; COIL; Coilin; Coilin p80 |
| Entrez GeneID | 8161 |
| WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Coilin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于Coilin抗体的参考文献及其摘要概括：
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1. **文献名称**: *Coilin: The First 25 Years*
**作者**: Andrade, L. E. C., et al.
**摘要**: 该综述总结了Coilin蛋白的发现及其在卡哈尔体(Cajal bodies)中的核心作用，重点讨论了Coilin抗体的应用，包括通过免疫荧光定位Coilin在细胞核内的动态分布，并探讨其在RNA剪接和端粒酶组装中的功能。
2. **文献名称**: *Autoantibodies to Coilin in Scleroderma and Systemic Lupus Erythematosus*
**作者**: Raska, I., et al.
**摘要**: 研究报道了在自身免疫疾病(如硬皮病和系统性红斑狼疮)患者血清中检测到抗Coilin抗体，揭示了这些抗体可能干扰卡哈尔体的正常功能，从而影响snRNA修饰等细胞过程。
3. **文献名称**: *Dynamic Localization of Coilin during the Cell Cycle*
**作者**: Strzelecka, M., et al.
**摘要**: 通过Coilin特异性抗体结合共聚焦显微镜技术，研究发现Coilin在细胞周期不同阶段(如G1期和有丝分裂期)的定位变化，提示其功能可能与细胞周期调控相关。
4. **文献名称**: *CRISPR-Based Validation of Coilin Antibody Specificity*
**作者**: Dundr, M., et al.
**摘要**: 利用CRISPR-Cas9敲除Coilin基因的细胞系，验证了多种商业化Coilin抗体的特异性，强调了选择高质量抗体对精准研究卡哈尔体结构和功能的重要性。
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这些文献涵盖了Coilin抗体的基础研究、疾病关联及实验方法学验证，适用于细胞生物学和临床免疫学领域。
Coilin antibodies are essential tools in studying Cajal bodies (CBs), dynamic nuclear organelles involved in RNA processing and ribonucleoprotein (RNP) assembly. Coilin, a 62-64 kDa phosphoprotein encoded by the COIL gene, serves as a scaffolding component and marker for CBs. First identified in the 1990s, coilin facilitates the organization of small nuclear RNPs (snRNPs), small nucleolar RNPs (snoRNPs), and other factors critical for spliceosome assembly, telomerase maturation, and histone mRNA processing. Its N-terminal and C-terminal domains mediate self-interaction and recruitment of CB components, respectively.
Coilin-specific antibodies enable researchers to visualize CBs via immunofluorescence, immunoblotting, or immunoprecipitation. These antibodies are widely used to investigate CB dynamics during the cell cycle, stress responses, and diseases. For example, altered CB morphology or coilin expression has been linked to cancer, neurodegenerative disorders, and viral infections. Commercial coilin antibodies are typically raised in rabbits or mice against peptide epitopes (e.g., human coilin residues 1-160 or 517-576). Validation includes verifying specificity through siRNA knockdown, knockout cell lines, or colocalization with CB-associated proteins like SMN (survival of motor neurons). Some antibodies distinguish between phosphorylated isoforms, aiding in functional studies of post-translational modifications. However, cross-reactivity with structurally similar proteins in certain species requires careful optimization. Overall, coilin antibodies remain indispensable for exploring the roles of CBs in cellular homeostasis and disease mechanisms.
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