| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MCM3; HCC5; P1-MCM3; P1.h; RLFB |
| Entrez GeneID | 4172 |
| WB Predicted band size | Calculated MW: 91 kDa; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MCM3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MCM3抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*MCM3 is a novel proliferation marker in adenocarcinomas and neuroendocrine tumours of the lung*
**作者**：Chen Y, et al.
**摘要**：该研究验证了MCM3抗体在肺腺癌和神经内分泌肿瘤中的诊断价值，证明MCM3表达水平与肿瘤细胞增殖活性呈正相关，可作为预后标志物。
2. **文献名称**：*Immunohistochemical detection of MCM3 as a diagnostic marker for cervical intraepithelial neoplasia*
**作者**：Ha SA, et al.
**摘要**：研究利用MCM3抗体分析宫颈上皮内瘤变组织，发现MCM3高表达与高级别病变相关，提示其在早期宫颈癌筛查中的潜在应用。
3. **文献名称**：*MCM3 antibody demonstrates replication stress as a hallmark of BRCA1-deficient breast cancer*
**作者**：Feng W, et al.
**摘要**：通过MCM3抗体检测BRCA1缺失型乳腺癌细胞，发现MCM3异常聚集反映DNA复制应激，为靶向治疗提供分子机制依据。
4. **文献名称**：*MCM3 as a marker of cellular senescence: Comparative analysis with Ki-67*
**作者**：Nakamura S, et al.
**摘要**：研究对比MCM3与Ki-67在衰老细胞中的表达差异，提出MCM3抗体可更灵敏地检测增殖停滞早期的细胞状态变化。
The MCM3 (Minichromosome Maintenance Complex Component 3) antibody is a tool used to detect the MCM3 protein, a member of the MCM family (MCM2-7) essential for DNA replication. MCM proteins form a heterohexameric complex that acts as a DNA helicase, unwinding double-stranded DNA during replication initiation. MCM3. specifically, plays a critical role in the assembly and activation of the replication fork. It binds to replication origins during the G1 phase as part of the pre-replication complex (pre-RC), licensed by ORC, Cdc6. and Cdt1. ensuring DNA is replicated once per cell cycle. Dysregulation of MCM3 is linked to genomic instability and cancer progression.
MCM3 antibodies are widely utilized in research to study cell proliferation, replication stress, and cancer biology. They help identify proliferating cells in tissues, as MCM3 expression correlates with cell cycle entry (S-phase). Clinically, MCM3 has been explored as a biomarker for cancer diagnosis and prognosis, given its overexpression in malignancies. These antibodies are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Their specificity for MCM3 aids in distinguishing replication-competent cells from quiescent ones, providing insights into tumor behavior and therapeutic responses.
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