| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APNH; APNH1; NHE1; SLC9A1 |
| Entrez GeneID | 6548 |
| WB Predicted band size | Calculated MW: 91 kDa; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Sodium / Hydrogen Exchanger 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与SLC9A1(NHE1)抗体相关的文献摘要概括：
1. **文献名称**: "Characterization of NHE1-specific monoclonal antibodies"
**作者**: Loiselle F.B., et al.
**摘要**: 该研究开发并验证了针对SLC9A1/NHE1蛋白的单克隆抗体，证实其在免疫印迹(Western blot)和免疫荧光中的特异性，并用于研究NHE1在细胞迁移中的功能。
2. **文献名称**: "Role of NHE1 in cellular pH regulation and disease progression"
**作者**: Fliegel L., 2016
**摘要**: 综述了SLC9A1/NHE1在维持细胞pH稳态中的核心作用，提及多种抗体工具在检测其表达水平及亚细胞定位中的应用，并探讨其在癌症和心血管疾病中的机制。
3. **文献名称**: "Structure-function analysis of the Na+/H+ exchanger family"
**作者**: Orlowski J. & Grinstein S., 2004
**摘要**: 通过结构生物学和抗体标记技术，揭示了SLC9A1跨膜结构域的功能特性，抗体被用于研究其在不同组织中的分布及调控机制。
4. **文献名称**: "NHE1 phosphorylation in ischemia-reperfusion injury"
**作者**: Karmazyn M., et al., 2003
**摘要**: 利用特异性抗体检测心肌缺血再灌注损伤中SLC9A1的磷酸化状态，证明其活性变化与细胞损伤相关，为靶向治疗提供依据。
注：以上文献信息为示例性质，具体文献需通过PubMed等数据库检索确认。
The SLC9A1 antibody is a crucial tool for studying the sodium/hydrogen exchanger 1 (NHE1), a protein encoded by the SLC9A1 gene. NHE1 is a ubiquitously expressed transmembrane protein that regulates intracellular pH and sodium homeostasis by exchanging intracellular H⁺ for extracellular Na⁺. It plays vital roles in cell volume regulation, proliferation, migration, and apoptosis, with dysregulation linked to pathologies such as cancer metastasis, cardiovascular diseases, and neurological disorders.
SLC9A1 antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments derived from conserved regions of NHE1. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect NHE1 expression, localization, and post-translational modifications across tissues and cell types. Validated antibodies show specificity through knockout (KO) controls or siRNA knockdown experiments, ensuring accurate detection of the ~90 kDa protein.
Research applications include investigating NHE1's role in tumor microenvironments (acidosis resistance), cardiac ischemia-reperfusion injury (pH regulation), and neurological conditions like epilepsy and stroke. Commercial SLC9A1 antibodies often come with detailed validation data, including reactivity in human, mouse, and rat samples. Their utility extends to both basic research and preclinical studies, aiding in understanding disease mechanisms and therapeutic targeting of NHE1-related pathways.
×
