| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1 |
| Entrez GeneID | 1642 |
| WB Predicted band size | Calculated MW: 127 kDa; Observed MW: 127 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DDB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及DDB1抗体的参考文献概览(注：文献为模拟示例，非真实存在)：
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1. **文献名称**：*DDB1 facilitates DNA damage response via mediating ubiquitination of p53*
**作者**：Chen L, et al.
**摘要**：研究利用DDB1特异性抗体进行免疫沉淀和Western blot，揭示DDB1通过调控p53泛素化参与DNA损伤修复的机制，证明DDB1缺失导致细胞对紫外线敏感。
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2. **文献名称**：*Antibody-based profiling of DDB1-CUL4 complex in carcinogenesis*
**作者**：Wang X, et al.
**摘要**：通过商业化DDB1抗体进行组织芯片免疫组化分析，发现DDB1在多种癌症中高表达，且与CUL4形成复合物促进肿瘤细胞增殖，提示其作为潜在治疗靶点。
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3. **文献名称**：*DDB1 knockdown impairs nucleotide excision repair in mammalian cells*
**作者**：Sugasawa K, et al.
**摘要**：使用DDB1抗体进行免疫荧光染色和ChIP实验，证实DDB1在细胞核内招募修复因子至DNA损伤位点，敲低DDB1显著降低核苷酸切除修复效率。
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注：以上内容为基于领域知识的模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。如需真实文献，建议以关键词“DDB1 antibody”或“DDB1 immunoblot”查询近年论文。
The DDB1 (Damage-specific DNA-binding protein 1) antibody is a critical tool for studying the DNA damage response and nucleotide excision repair (NER) pathways. DDB1. a 127 kDa protein, forms a heterodimer with DDB2 to create the UV-DDB complex, which recognizes UV-induced DNA lesions, such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts. This complex facilitates the recruitment of XPC, initiating global genome NER. Beyond its role in DNA repair, DDB1 functions as a core component of the CUL4A/B-RBX1-DDB1 E3 ubiquitin ligase complex, regulating ubiquitination and proteasomal degradation of target proteins involved in cell cycle control, chromatin remodeling, and transcription.
DDB1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. They are essential for investigating DDB1's dual roles in DNA repair and ubiquitination-mediated signaling, as well as its association with diseases, including xeroderma pigmentosum (XP) and cancers linked to genomic instability. Commercial DDB1 antibodies are typically raised in rabbits or mice, validated for specificity using knockout cell lines or siRNA knockdown. Studies utilizing these antibodies have clarified mechanisms underlying UV-induced mutagenesis, chemotherapy resistance, and the crosstalk between DNA repair and epigenetic regulation. Their application continues to advance understanding of genomic maintenance systems and therapeutic targeting of DNA repair-deficient cancers.
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