| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TXN; MTRX; TRX2; MT-TRX; COXPD29 |
| Entrez GeneID | 25828 |
| WB Predicted band size | Calculated MW: 18 kDa; Observed MW: 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Thioredoxin 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Thioredoxin 2(Trx2)抗体的参考文献示例(注：文献信息为虚构示例，仅用于演示格式)：
1. **文献名称**：*Mitochondrial Thioredoxin-2 regulates oxidative stress-induced apoptosis*
**作者**：Smith J, et al.
**摘要**：本研究利用Trx2特异性抗体，通过Western blot和免疫荧光技术，揭示了Trx2在线粒体氧化应激中对细胞凋亡的调控作用，表明其缺失导致活性氧(ROS)积累和线粒体膜电位崩溃。
2. **文献名称**：*Thioredoxin-2 antibody-based detection in neurodegenerative disease models*
**作者**：Lee S, et al.
**摘要**：通过Trx2抗体在小鼠脑组织中的免疫组化分析，发现Trx2表达水平在阿尔茨海默病模型中显著降低，提示其与神经退行性病变中线粒体功能障碍相关。
3. **文献名称**：*Characterization of Trx2 knockout mice using specific polyclonal antibodies*
**作者**：Garcia R, et al.
**摘要**：开发并验证了一种高特异性Trx2多克隆抗体，用于检测基因敲除小鼠模型中线粒体Trx2的蛋白表达缺失，证实Trx2在胚胎发育中的必要性。
如需真实文献，建议在PubMed或Google Scholar检索关键词：“Thioredoxin 2 antibody”或“Trx2 antibody”，筛选实验方法部分明确提及该抗体的研究。
Thioredoxin 2 (Trx2) is a mitochondrial-specific redox protein belonging to the thioredoxin family, which plays a critical role in maintaining cellular redox homeostasis. It regulates antioxidant defense by scavenging reactive oxygen species (ROS) and reducing oxidized proteins via its conserved CXXC catalytic motif. Trx2 is essential for mitochondrial function, influencing processes such as apoptosis, cell proliferation, and energy metabolism. It interacts with mitochondrial proteins like apoptosis signal-regulating kinase 1 (ASK1) and modulates signaling pathways linked to oxidative stress responses. Dysregulation of Trx2 has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer, highlighting its importance in cellular health.
Antibodies targeting Trx2 are vital tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies enable detection of Trx2 in techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in research on mitochondrial oxidative stress mechanisms. They also help assess Trx2’s interaction partners and post-translational modifications, such as nitrosylation or oxidation, which regulate its activity. Validated Trx2 antibodies are crucial for ensuring specificity and reliability in experimental models, from cell cultures to animal tissues. Their application advances understanding of mitochondrial dysfunction in aging and disease, offering insights into therapeutic strategies targeting redox imbalance.
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