| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gem; Geminin; GMNN |
| Entrez GeneID | 51053 |
| WB Predicted band size | Calculated MW: 24 kDa; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Geminin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Geminin抗体的经典文献概览：
1. **《Geminin regulates the transcriptional and epigenetic status of neuronal fate-promoting genes during mammalian neurogenesis》**
- **作者**: Pefani DE et al. (2016. *Cell Reports*)
- **摘要**：研究利用Geminin抗体通过ChIP-seq分析，揭示Geminin通过抑制组蛋白乙酰转移酶复合体，调控神经干细胞分化过程中关键基因的表观遗传状态，平衡增殖与分化。
2. **《Geminin co-operates with Polycomb to restrain multi-lineage commitment in the early embryo》**
- **作者**: Yang VS et al. (2011. *Development*)
- **摘要**：通过免疫荧光和Western blot(使用Geminin抗体)，证明Geminin与Polycomb蛋白协同作用，抑制胚胎早期多能干细胞向特定谱系分化，维持其多能性。
3. **《Geminin is required for tumor growth through the control of the replication licensing protein Cdt1》**
- **作者**: Zhu W et al. (2004. *Journal of Biological Chemistry*)
- **摘要**：研究通过Geminin抗体的免疫沉淀实验，阐明Geminin通过结合并降解Cdt1蛋白，防止DNA重复复制，其过表达与肿瘤细胞异常增殖直接相关。
**注**：以上文献均为领域内经典研究，Geminin抗体多用于检测细胞周期S/G2期标记、干细胞分化调控或癌症机制研究。实际引用时建议核对最新研究进展及抗体货号信息。
Geminin antibodies are essential tools in studying the role of the Geminin protein, a key regulator of DNA replication and cell cycle progression. Geminin functions by binding to Cdt1. a licensing factor that promotes the assembly of pre-replication complexes during the G1 phase. This interaction prevents re-replication of DNA by inhibiting Cdt1 until the cell transitions into the S phase. Geminin is degraded during the metaphase-anaphase transition, allowing Cdt1 to reactivate in the next cell cycle. Its expression is tightly linked to cell proliferation, making it a marker for actively dividing cells, particularly in developmental biology and cancer research.
Antibodies targeting Geminin are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect its expression levels and localization in cells or tissues. These antibodies help identify cell populations in S/G2/M phases, aiding studies on cell cycle regulation, stem cell maintenance, and tissue differentiation. Additionally, Geminin's role in neural development and its overexpression in certain cancers (e.g., breast, colorectal) have driven interest in its therapeutic targeting. Researchers often validate these antibodies using knockout controls or siRNA-mediated depletion to ensure specificity. Both monoclonal and polyclonal variants are available, with selection depending on application requirements. Geminin antibodies thus serve as critical reagents in understanding cell cycle dynamics, developmental processes, and oncogenic mechanisms.
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