| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1 |
| Entrez GeneID | 3516 |
| WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RBPJK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBP-Jκ(RBPJK)抗体的3篇代表性文献摘要：
1. **文献名称**：*RBP-J (RBP-κ) regulates cell proliferation and exit from the naïve pluripotent state in mouse embryonic stem cells*
**作者**：Shi X, et al.
**摘要**：该研究利用RBP-Jκ特异性抗体通过ChIP-seq和免疫沉淀技术，揭示RBP-Jκ通过调控Notch信号通路靶基因，影响小鼠胚胎干细胞的增殖和干性维持。研究验证了抗体在Western blot和免疫荧光中的特异性应用。
2. **文献名称**：*RBP-J suppresses mammary tumorigenesis through transcriptional regulation of Notch signaling components*
**作者**：Kang J, et al.
**摘要**：通过RBP-Jκ抗体进行组织免疫组化分析，发现其在乳腺癌中低表达，并证实其通过抑制Notch下游靶点(如Hes1)抑制肿瘤进展。研究强调了抗体在临床样本中的诊断潜力。
3. **文献名称**：*RBP-J-dependent Notch signaling regulates retinal progenitor cell fate determination*
**作者**：Zhang L, et al.
**摘要**：使用RBP-Jκ抗体进行视网膜发育研究，发现条件性敲除模型中RBP-Jκ缺失导致神经前体细胞分化异常。抗体被用于流式细胞术和共聚焦成像，证实其跨物种反应性(小鼠/斑马鱼)。
注：RBP-Jκ抗体常用研究领域集中于Notch通路调控的细胞分化、癌症及干细胞生物学，上述文献均通过实验验证了抗体的特异性(如基因敲除对照)。实际引用时建议补充具体年份及期刊。
The Recombination Signal Binding Protein for Immunoglobulin Kappa J Region (RBPJ), also known as CBF1 or CSL, is a highly conserved DNA-binding transcription factor central to the canonical Notch signaling pathway. RBPJ serves as a critical mediator of Notch signaling, a key pathway regulating cell fate determination, differentiation, and development. In the absence of Notch activation, RBPJ typically acts as a transcriptional repressor by recruiting co-repressor complexes. Upon Notch receptor-ligand interaction, the intracellular domain of Notch (NICD) translocates to the nucleus, displaces co-repressors, and forms a complex with RBPJ to activate transcription of target genes like Hes and Hey families.
RBPJ antibodies are essential tools for studying Notch signaling dynamics in developmental biology, cancer research, and stem cell regulation. They enable detection of RBPJ expression, localization, and DNA-binding activity through techniques including Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and electrophoretic mobility shift assays (EMSA). Aberrant RBPJ/Notch signaling is implicated in various diseases, including T-cell leukemia, cardiovascular disorders, and neurological conditions, making these antibodies valuable for mechanistic and therapeutic investigations. Commercial RBPJ antibodies are typically raised against conserved epitopes, with validation across human, mouse, and rat models to support cross-species studies.
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