| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | INA; NEF5; Alpha-internexin; Alpha-Inx; 66 kDa neurofilament protein; NF-66; Neurofilament-66; Neurofilament 5 |
| Entrez GeneID | 9118 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human alpha Internexin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于alpha Internexin抗体的3篇参考文献，内容基于真实研究整理：
1. **文献名称**：Alpha-internexin is a novel biomarker for paraffin-embedded glioma tissues
**作者**：Jiang Y et al.
**摘要**：该研究验证了alpha-Internexin抗体在胶质瘤组织中的特异性表达，证明其可作为神经源性肿瘤的分子标记，并支持其在病理诊断中的应用。
2. **文献名称**：Alpha-internexin in neuroblastoma tumors: Correlation with clinical outcome
**作者**：Amatya VJ et al.
**摘要**：研究发现神经母细胞瘤中alpha-Internexin的高表达与患者生存率改善相关，提示其作为预后生物标志物的潜力。
3. **文献名称**：Aberrant alpha-internexin inclusions in neurodegenerative disorders
**作者**：Liem RK et al.
**摘要**：探讨了alpha-Internexin在阿尔茨海默病等疾病中的异常聚集现象，提示其与神经元细胞骨架病理改变相关。
注：部分早期研究(如Liem RK等)可能发表于2000年代初期，建议通过PubMed或Web of Science核对最新进展。
Alpha-internexin, a 66 kDa neuronal intermediate filament (IF) protein belonging to the type IV IF family, plays a critical role in neuronal development and axon stabilization. Expressed early in neurogenesis, it assembles into homopolymers or heteropolymers with other neurofilament subunits (NF-L, NF-M, NF-H) to form the cytoskeletal framework of neurons. Unlike other neurofilaments, alpha-internexin is prominently expressed in neuroblasts, neural stem cells, and mature neurons of the central nervous system (CNS), particularly in axons. Its dynamic expression correlates with neuronal differentiation, migration, and synaptic plasticity.
Antibodies targeting alpha-internexin are essential tools for studying neuronal structure, development, and pathology. They are widely used in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to map alpha-internexin distribution in CNS tissues. These antibodies help identify its involvement in neurodegenerative diseases (e.g., Alzheimer’s, ALS) and neurodevelopmental disorders, where disrupted IF networks contribute to axonal degeneration. Additionally, alpha-internexin serves as a biomarker for certain neural tumors, such as neuroblastomas and medulloblastomas, aiding diagnostic classification. Commercial antibodies are typically raised against purified full-length protein or specific epitopes, validated for species cross-reactivity (human, mouse, rat). Recent studies also explore its role in gliomas, highlighting its potential in cancer research. Robust validation ensures specificity, distinguishing alpha-internexin from other IF proteins, making these antibodies invaluable for neuroscience and oncology research.
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