| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADRBK2; BARK2; GRK3 |
| Entrez GeneID | 157 |
| WB Predicted band size | Calculated MW: 80 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GRK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GRK3抗体的3篇参考文献示例(注：以下内容为模拟虚构，仅用于格式参考，实际文献需通过数据库检索获取)：
1. **文献名称**：*"GRK3 Expression in Metastatic Breast Cancer: Role in Cell Migration and Therapeutic Implications"*
**作者**：Smith A., et al.
**摘要**：该研究通过免疫组化及Western blot分析，发现GRK3在转移性乳腺癌组织中高表达，并与细胞迁移能力增强相关。使用特异性GRK3抗体验证其作为潜在治疗靶点的可行性。
2. **文献名称**：*"Development and Validation of a Novel GRK3-Specific Antibody for Neurodegenerative Disease Studies"*
**作者**：Chen L., et al.
**摘要**：研究团队开发了一种高特异性GRK3单克隆抗体，并通过免疫荧光和ELISA验证其灵敏度。该抗体成功用于检测阿尔茨海默病模型中GRK3的异常表达。
3. **文献名称**：*"GRK3 Modulates Dopamine Receptor Signaling in Schizophrenia: Insights from Antibody-Based Profiling"*
**作者**：Kim J., et al.
**摘要**：通过GRK3抗体检测精神分裂症患者脑组织样本，发现GRK3表达水平与多巴胺受体信号通路的失调显著相关，提示其参与疾病病理机制。
如需真实文献，建议通过PubMed或Google Scholar检索关键词：**GRK3 antibody**, **G protein-coupled receptor kinase 3**, 并筛选应用类研究。
**Background of GRK3 Antibody**
G protein-coupled receptor kinase 3 (GRK3), also known as β-adrenergic receptor kinase 2 (βARK2), is a member of the GRK family that regulates G protein-coupled receptor (GPCR) signaling by phosphorylating activated receptors, leading to their desensitization and internalization. GRK3 is widely expressed in various tissues, including the brain, heart, and immune cells, and plays a critical role in modulating physiological processes such as neurotransmission, cardiac function, and immune responses. Dysregulation of GRK3 has been implicated in multiple pathologies, including heart failure, psychiatric disorders (e.g., schizophrenia and bipolar disorder), and cancer progression.
GRK3 antibodies are essential tools for studying the expression, localization, and functional roles of GRK3 in both normal and diseased states. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect GRK3 protein levels in tissues or cells. Researchers also employ GRK3 antibodies to investigate interactions with GPCRs or downstream signaling molecules, aiding in the exploration of therapeutic targets. For example, GRK3 inhibition has been proposed as a strategy to enhance β-adrenergic receptor signaling in heart failure or to modulate chemokine receptor activity in cancer metastasis.
Commercial GRK3 antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or kinase domains). Validation for specificity (via knockout controls) and application compatibility is critical due to potential cross-reactivity with other GRK isoforms (e.g., GRK2). Ongoing research continues to clarify GRK3's diverse roles, driving demand for reliable antibodies in mechanistic and translational studies.
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