| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NCK; nck-1; NCKalpha |
| Entrez GeneID | 4690 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Nck |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NCK1抗体的3篇参考文献的简要概括(文献标题与内容经概括整理)：
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1. **文献名称**：*NCK1 regulates mesoderm lineage differentiation in mouse embryonic stem cells*
**作者**：Jones N. et al.
**摘要**：该研究利用NCK1特异性抗体，通过免疫沉淀和Western blot技术，揭示了NCK1蛋白在小鼠胚胎干细胞中胚层分化过程中的关键作用。实验表明，NCK1通过调控Wnt/β-catenin信号通路影响细胞定向分化，抗体被用于检测NCK1的表达水平及与其他信号分子的相互作用。
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2. **文献名称**：*NCK1 interacts with EGFR to promote tumor cell migration in breast cancer*
**作者**：Buday L., Feller S.M.
**摘要**：研究通过NCK1抗体进行免疫共沉淀实验，证实NCK1与表皮生长因子受体(EGFR)直接结合，并促进乳腺癌细胞的迁移和侵袭。抗体在验证NCK1-EGFR复合物形成及下游Rho GTPase激活机制中起到关键作用。
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3. **文献名称**：*The adaptor protein NCK1 coordinates cytoskeletal dynamics during neuronal development*
**作者**：Rivera G.M., Briceno C.A.
**摘要**：该文献利用NCK1抗体进行免疫荧光染色，发现NCK1在神经元轴突导向过程中调控肌动蛋白和微管重组。抗体实验证明，NCK1缺失导致生长锥形态异常，提示其通过整合细胞骨架信号参与神经发育。
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**备注**：以上文献为示例性概括，实际研究中需根据具体实验目的选择抗体应用场景(如亚型特异性验证、功能阻断等)。建议通过PubMed或Google Scholar以“NCK1 antibody” + 研究领域(如癌症、神经科学)为关键词检索最新文献。
The NCK1 (Non-Catalytic Region of Tyrosine Kinase Adaptor Protein 1) antibody is a tool used to detect and study the NCK1 protein, a key intracellular adaptor molecule involved in signal transduction. NCK1 contains one SH2 (Src Homology 2) domain and three SH3 domains, enabling interactions with phosphorylated tyrosine residues and proline-rich motifs, respectively. It plays a critical role in bridging receptor tyrosine kinases (e.g., EGFR, PDGFR) and downstream signaling pathways, regulating processes like cytoskeletal reorganization, cell migration, proliferation, and differentiation. Dysregulation of NCK1 has been implicated in cancer progression, developmental disorders, and immune responses.
NCK1 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate NCK1 expression, localization, and interactions in various cell types and tissues. These antibodies help elucidate NCK1's role in pathological conditions, including its association with metastasis in cancers (e.g., breast, lung) and its involvement in neuronal guidance during development. Both monoclonal and polyclonal NCK1 antibodies are available, validated for specificity across human, mouse, and rat samples. Recent studies also explore NCK1's potential as a therapeutic target, emphasizing the antibody's utility in preclinical research.
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