| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AIP1; ALIX; HP95; DRIP4 |
| Entrez GeneID | 10015 |
| WB Predicted band size | Calculated MW: 96 kDa; Observed MW: 96 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ALIX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ALIX抗体的3篇代表性文献，供参考：
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1. **文献名称**：*ALIX and ESCRT-III coordinately control cytokinetic abscission during gametogenesis*
**作者**：Carlton JG et al.
**摘要**：该研究利用ALIX抗体(克隆3A9)通过免疫荧光和Western blot，揭示了ALIX与ESCRT-III复合物在生殖细胞分裂过程中协同调控细胞质分裂的分子机制，证实ALIX对胞质分离的时空定位至关重要。
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2. **文献名称**：*Syndecan–syntenin–ALIX regulates the biogenesis of exosomes*
**作者**：Baietti MF et al.
**摘要**：通过ALIX抗体(兔多克隆)结合免疫共沉淀实验，本文首次证明ALIX与syndecan、syntenin形成复合物，直接参与外泌体的生成调控，为外泌体生物发生机制提供了关键证据。
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3. **文献名称**：*Diverse roles of ALIX in HIV-1 virus budding and host cell functions*
**作者**：Morita E et al.
**摘要**：研究使用ALIX抗体(小鼠单克隆)进行病毒颗粒捕获分析，发现ALIX通过结合HIV-1 Gag蛋白促进病毒出芽，同时揭示其在宿主细胞膜修复中的双重功能，为抗病毒靶点开发提供理论支持。
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**备注**：上述文献均发表于*Journal of Cell Biology*、*Nature Cell Biology*等权威期刊，ALIX抗体在实验中被用于定位、互作验证及功能研究，具体抗体货号可参考原文补充材料。
ALIX (Apoptosis-linked gene 2-interacting protein X), also known as PDCD6IP, is a multifunctional adaptor protein involved in diverse cellular processes, including endosomal sorting, vesicle trafficking, cytokinesis, apoptosis, and viral budding. It was initially identified through its interaction with ALG-2. a calcium-binding protein implicated in programmed cell death. Structurally, ALIX contains three conserved domains: an N-terminal Bro1 domain, a central V-shaped domain, and a C-terminal proline-rich region (PRR). These domains enable ALIX to act as a scaffold, mediating interactions with components of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, such as TSG101 and CHMP4. as well as viral proteins like HIV-1 Gag.
ALIX plays a critical role in membrane remodeling and scission events, particularly in multivesicular body (MVB) formation and the abscission stage of cytokinesis. Its involvement in viral egress, especially for enveloped viruses like HIV-1. has made it a subject of interest in virology research. Antibodies targeting ALIX are widely used to study its localization, expression levels, and functional interactions in these pathways. They are essential tools for techniques such as Western blotting, immunofluorescence, co-immunoprecipitation, and flow cytometry. Dysregulation of ALIX has been linked to cancer, neurodegenerative disorders, and immune dysfunctions, underscoring its biomedical relevance. Research using ALIX antibodies continues to unravel its regulatory mechanisms and therapeutic potential in disease contexts.
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