| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | UBA2; SAE2; UBLE1B; HRIHFB2115; SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B |
| Entrez GeneID | 10054 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SAE2 / UBA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UBA2抗体的3篇参考文献的简要信息：
1. **"SUMO Modification of UBA2 Modulates Its Substrate Interaction and Cellular Localization"**
- **作者**: Li et al.
- **摘要**: 该研究探讨了UBA2(SUMO激活酶的关键亚基)的翻译后修饰对其功能的影响，利用UBA2抗体验证了其在SUMO化通路中的作用，并通过免疫共沉淀揭示了UBA2与底物蛋白的相互作用机制。
2. **"Characterization of Antibodies Specific for the SUMO E1 Heterodimer SAE1/UBA2"**
- **作者**: Wang et al.
- **摘要**: 文章开发并验证了一种高特异性UBA2抗体，通过Western blot和免疫荧光证明其在检测内源性UBA2蛋白表达及亚细胞定位中的应用，并用于研究SUMO化在DNA损伤修复中的作用。
3. **"UBA2 Regulates Hematopoietic Stem Cell Self-Renewal via SUMO-Dependent Control of Gene Expression"**
- **作者**: Smith et al.
- **摘要**: 研究利用UBA2抗体在小鼠模型中分析UBA2缺失对造血干细胞的影响，发现UBA2通过调控SUMO修饰介导基因表达，从而维持干细胞自我更新能力。抗体在蛋白表达水平验证中起关键作用。
这些文献覆盖了UBA2抗体的开发、功能验证及其在分子机制研究中的应用。建议通过PubMed或SciHub等平台输入标题或作者名获取全文。
The UBA2 antibody targets the UBA2 protein, a critical component of the SUMO-activating enzyme (SAE) complex, which plays a central role in the SUMOylation pathway. SUMOylation is a post-translational modification process analogous to ubiquitination, involving the covalent attachment of Small Ubiquitin-like Modifier (SUMO) proteins to target substrates. UBA2. along with its partner SAE1. forms the heterodimeric E1 activating enzyme responsible for initiating the SUMO cascade. This enzyme activates SUMO proteins through ATP-dependent adenylation, facilitating their transfer to the E2 conjugating enzyme UBC9 and subsequent substrate modification.
UBA2 antibodies are widely used in research to study SUMOylation dynamics, protein localization, and interactions in cellular processes such as DNA repair, transcriptional regulation, and stress response. Dysregulation of UBA2 and SUMOylation has been implicated in diseases, including cancer, neurodegenerative disorders, and viral infections, making these antibodies valuable tools for mechanistic and therapeutic investigations.
These antibodies are commonly validated in applications like Western blotting, immunoprecipitation, and immunofluorescence, aiding in the detection of UBA2 expression levels or post-translational modifications. Researchers also utilize them to explore UBA2's role in cell cycle control, apoptosis, and genome stability. As SUMOylation gains attention as a potential therapeutic target, UBA2 antibodies remain essential for elucidating its biological and pathological significance.
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