| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC46; MCM5; MGC5315; P1 CDC46 |
| Entrez GeneID | 4174 |
| WB Predicted band size | Calculated MW: 82 kDa; Observed MW: 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human MCM5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MCM5抗体的3篇参考文献概览：
1. **文献名称**：*Mini-chromosome maintenance protein 5 as a biomarker for bladder cancer detection in urine*
**作者**：Stoeber, K., et al. (2001)
**摘要**：该研究利用MCM5特异性抗体，通过免疫细胞化学技术检测尿液中脱落细胞的MCM5表达水平。结果显示，膀胱癌患者尿液中MCM5阳性细胞显著增多，提示其作为无创诊断工具的潜力。
2. **文献名称**：*MCM5 expression in cervical precancerous lesions: A potential biomarker for early diagnosis*
**作者**：Williams, G.H., et al. (2005)
**摘要**：通过免疫组化技术结合MCM5抗体，研究发现宫颈高度鳞状上皮内病变(HSIL)中MCM5蛋白表达显著升高，表明其在宫颈癌前病变筛查中的临床价值。
3. **文献名称**：*Development of a novel ELISA assay for MCM5 in colorectal cancer using monoclonal antibodies*
**作者**：Nishihara, K., et al. (2018)
**摘要**：研究团队开发了一种基于MCM5单克隆抗体的ELISA检测方法，用于定量结直肠癌患者血清中的MCM5水平。该方法显示出高灵敏度和特异性，为癌症早期诊断提供了新途径。
以上文献均聚焦于MCM5抗体的应用，涵盖尿液检测、组织病理学诊断及血清学技术开发，突显其在肿瘤标志物研究中的多场景潜力。
The MCM5 (Minichromosome Maintenance Complex Component 5) antibody is a key tool for studying the MCM protein family, which plays a critical role in DNA replication initiation and cell cycle regulation. As part of the hexameric MCM2-7 helicase complex, MCM5 is essential for forming pre-replication complexes during the G1 phase, ensuring precise duplication of genomic material in S phase. Its expression is tightly cell cycle-dependent, peaking during proliferation, making it a biomarker for actively dividing cells.
MCM5 antibodies are widely used in research to investigate DNA replication mechanisms, cell cycle progression, and genomic stability. In diagnostic and clinical contexts, they help detect dysregulated cell proliferation, as MCM5 overexpression has been observed in various cancers (e.g., colorectal, cervical, and prostate cancers). Unlike traditional proliferation markers like Ki-67. MCM5 may better identify early-stage malignant transformations due to its specific expression pattern in replicating cells.
These antibodies are routinely applied in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Recent studies explore their potential in liquid biopsies for cancer screening and monitoring therapeutic responses. However, challenges remain in standardizing detection thresholds across tissue types and distinguishing benign hyperplasia from malignancy. Ongoing research continues to refine MCM5's utility in both basic science and translational oncology applications.
×
