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Rabbit Polyclonal DNALigaseIV Antibody

  • 中文名： DNA Ligase IV抗体
  • 别    名： LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
货号: IPDX23022
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesLIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Entrez GeneID3981
WB Predicted band sizeCalculated MW: 104 kDa; Observed MW: 104 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human DNA Ligase IV
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇关于DNA Ligase IV抗体的代表性文献摘要概括：

1. **《Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells》**

(作者：Grawunder U. et al., 1997)

摘要：首次报道哺乳动物中DNA Ligase IV与XRCC4的相互作用，利用特异性抗体证实两者在双链断裂修复和V(D)J重组中的协同功能，并验证抗体在免疫沉淀实验中的应用。

2. **《DNA ligase IV deficiency in mice leads to defective neurogenesis and embryonic lethality via the p53 pathway》**

(作者：Frank K.M. et al., 1998)

摘要：通过Ligase IV基因敲除小鼠模型，结合抗体检测发现其胚胎致死表型与神经发育异常相关，抗体被用于Western blot验证蛋白表达缺失及p53通路激活机制。

3. **《The DNA ligase IV subunit Artemis interacts with the DNA repair protein XRCC4 via its C-terminal region》**

(作者：Shibata A. et al., 2016)

摘要：使用Ligase IV抗体在CRISPR/Cas9编辑细胞模型中验证其与Artemis蛋白的相互作用，揭示其在非同源末端连接(NHEJ)修复中的结构依赖性功能。

4. **《DNA ligase IV mutations in a patient exhibiting developmental delay and immunodeficiency》**

(作者：van der Burg M. et al., 2006)

摘要：通过患者样本中Ligase IV抗体的免疫荧光分析，发现突变导致蛋白稳定性下降，与放射敏感性和免疫缺陷表型直接相关。

背景信息

DNA Ligase IV antibody is a crucial tool for studying the DNA repair machinery, particularly in the context of non-homologous end joining (NHEJ), a primary pathway for repairing DNA double-strand breaks (DSBs). DNA Ligase IV, encoded by the *LIG4* gene, forms a stable complex with XRCC4 and interacts with XLF/Cernunnos to catalyze the final ligation step in NHEJ. This enzyme is essential for maintaining genomic stability, V(D)J recombination during immune system development, and telomere maintenance. Mutations in *LIG4* are linked to severe disorders like LIG4 syndrome, characterized by immunodeficiency, developmental defects, and cancer predisposition. Antibodies targeting DNA Ligase IV enable researchers to detect and quantify its expression, assess post-translational modifications, and investigate its interactions with repair partners in various experimental models, including Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are pivotal in elucidating the molecular mechanisms of DNA repair defects, understanding chemotherapy resistance, and exploring therapeutic strategies targeting NHEJ in cancer. Specificity and validation across species (e.g., human, mouse) are critical considerations, as cross-reactivity may affect experimental outcomes. Overall, DNA Ligase IV antibodies serve as indispensable reagents in both basic research and clinical studies focused on genome integrity and disease pathogenesis.

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