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Rabbit Polyclonal ULK3 Antibody

  • 中文名： ULK3抗体
  • 别    名： Serine/threonine-protein kinase ULK3; Ulk3; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3
货号: IPDX22982
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSerine/threonine-protein kinase ULK3; Ulk3; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3
Entrez GeneID25989
WB Predicted band sizeCalculated MW: 53 kDa; Observed MW: 53 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human ULK3
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于ULK3抗体的3篇参考文献示例(注：以下内容为示例性概括，具体文献需根据实际数据库检索确认)：

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1. **文献名称**：*"ULK3 modulates autophagic flux through its kinase activity and interaction with ATG13"*

**作者**：Chen Y, et al.

**摘要**：该研究通过开发特异性ULK3抗体，验证了ULK3在自噬体形成中的作用。作者利用Western blot和免疫荧光技术，发现ULK3通过与ATG13的相互作用调控自噬通量，其激酶活性对饥饿诱导的自噬至关重要。

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2. **文献名称**：*"Characterization of ULK3 expression in human cancers using a novel monoclonal antibody"*

**作者**：Smith J, et al.

**摘要**：研究团队开发了一种高特异性ULK3单克隆抗体，并用于分析多种癌症组织中ULK3的蛋白表达水平。结果显示，ULK3在肺癌和乳腺癌中显著上调，且与患者预后不良相关，提示其可能作为潜在治疗靶点。

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3. **文献名称**：*"ULK3 regulates TGF-β signaling by phosphorylating SMAD2/3 in fibroblasts"*

**作者**：Lee S, et al.

**摘要**：通过免疫共沉淀(使用ULK3抗体)和激酶活性实验，研究发现ULK3直接磷酸化SMAD2/3蛋白，从而增强TGF-β信号通路在成纤维细胞中的功能，为纤维化疾病的机制提供了新见解。

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**注意**：以上文献信息为示例，实际引用时建议通过PubMed或Google Scholar以“ULK3 antibody”、“ULK3 kinase”等关键词检索真实文献。部分研究可能未在摘要中明确提及抗体使用细节，需结合全文方法部分确认。

背景信息

The ULK3 (Unc-51-like kinase 3) antibody is a research tool designed to detect and study the ULK3 protein, a serine/threonine kinase belonging to the Unc-51 family. ULK3 shares structural homology with ULK1 and ULK2. key regulators of autophagy initiation, but its precise biological roles remain less defined. It contains an N-terminal kinase domain and a C-terminal regulatory domain, with potential roles in cell signaling, differentiation, and stress responses. ULK3 has been implicated in pathways such as Hedgehog signaling, where it phosphorylates Gli transcription factors, and in autophagy regulation under specific conditions, though its mechanisms may diverge from ULK1/2. Dysregulation of ULK3 is associated with diseases like cancer and fibrosis, making it a target for mechanistic and therapeutic exploration. The antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ULK3 expression, localization, and post-translational modifications. Researchers also employ it to study interactions with binding partners (e.g., ATG13) and its responsiveness to cellular cues like nutrient deprivation or mTOR/AMPK pathway modulation. Validation of antibody specificity via knockout controls is critical, as ULK3’s homology with other kinases may lead to cross-reactivity. Ongoing studies aim to clarify its context-dependent roles in development and disease.

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