| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDT 1; Chromatin licensing and DNA replication factor 1; Retroviral integration site 2; RIS2 |
| Entrez GeneID | 81620 |
| WB Predicted band size | Calculated MW: 60 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CDT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDT1抗体的3篇参考文献的简要总结(注：文献信息为模拟示例，非真实存在)：
1. **文献名称**：*CDT1 phosphorylation by cyclin A-dependent kinases regulates DNA replication in human cells*
**作者**：J. Xiong, et al.
**摘要**：研究通过CDT1抗体检测其在细胞周期中的磷酸化状态，发现Cyclin A依赖的激酶调控CDT1活性以阻止DNA重复复制，确保基因组稳定性。
2. **文献名称**：*Overexpression of CDT1 in cancer cells correlates with poor prognosis*
**作者**：H. Nishitani, et al.
**摘要**：利用CDT1抗体分析多种癌症组织样本，发现CDT1在肿瘤细胞中异常高表达，且与患者生存率下降相关，提示其作为癌症生物标志物的潜力。
3. **文献名称**：*CUL4-DDB1 ubiquitin ligase interacts with CDT1 to regulate DNA replication licensing*
**作者**：L. Zhong, et al.
**摘要**：通过免疫共沉淀(使用CDT1抗体)揭示CUL4-DDB1复合物介导CDT1的泛素化降解，调控复制起始复合体的组装，维持细胞周期正常进程。
(注：以上文献为示例性内容，实际引用请查询权威数据库如PubMed。)
The CDT1 antibody is a crucial tool in studying the regulation of DNA replication and cell cycle progression. CDT1 (Cdc10-dependent transcript 1) is a conserved protein essential for initiating DNA replication by loading the mini-chromosome maintenance (MCM) complex onto replication origins during the G1 phase. Its activity is tightly regulated to prevent re-replication, a key mechanism maintaining genomic stability. CDT1 levels peak in G1. decline during S phase through CRL4-CDT2-mediated ubiquitination, and remain low until the next cycle. Dysregulation of CDT1 is linked to genomic instability, cancer, and developmental disorders.
CDT1 antibodies are widely used in research to detect and quantify CDT1 expression, assess its cell cycle-dependent fluctuations, and investigate interactions with licensing factors (e.g., CDC6. geminin) or degradation pathways. They are employed in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Studies using these antibodies have elucidated CDT1's role in replication stress, oncogene activation, and chemotherapy resistance. Additionally, they help identify pathological conditions marked by CDT1 overexpression, such as certain cancers, providing insights into diagnostic or therapeutic strategies. Commercial CDT1 antibodies are typically validated for specificity across human, mouse, and rat samples, ensuring broad experimental applicability.
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