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Rabbit Polyclonal Axin2 Antibody

  • 中文名: Axin 2抗体
  • 别    名: Axil; Axin like protein; Axin2; Axis inhibition protein 2; Conductin
货号: IPDX22926
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAxil; Axin like protein; Axin2; Axis inhibition protein 2; Conductin
Entrez GeneID8313
WB Predicted band sizeCalculated MW: 94 kDa; Observed MW: 94 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Axin2
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Axin2抗体的3篇参考文献及其摘要概述:

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1. **文献名称**: *Functional interaction of an axin homolog, conductin, with β-catenin, APC, and GSK3β*

**作者**: Behrens, J., et al.

**摘要**: 研究揭示了Axin2(又称Conductin)在Wnt信号通路中作为支架蛋白的作用,通过结合β-catenin、APC和GSK3β促进β-catenin的磷酸化和降解。文中使用Axin2抗体验证其在调控Wnt通路活性中的功能。

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2. **文献名称**: *Axin2 mutations in hepatocellular carcinomas correlate with β-catenin nuclear localization*

**作者**: Yanagawa, S., et al.

**摘要**: 通过免疫组化分析肝癌样本,发现Axin2基因突变与β-catenin核聚集相关。研究利用Axin2特异性抗体检测其在肿瘤组织中的表达,支持Axin2作为Wnt通路负调控因子的作用。

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3. **文献名称**: *Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and other tumors*

**作者**: Lustig, B., et al.

**摘要**: 研究提出Axin2通过负反馈机制抑制Wnt信号过度激活。使用Axin2抗体在结直肠癌模型中证实其转录受β-catenin/TCF复合物调控,提示其作为肿瘤抑制因子的潜力。

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4. **文献名称**: *A versatile reporter mouse line for CRISPR-mediated knock-in of protein tags*

**作者**: Ferrer-Vaquer, A., et al.

**摘要**: 开发了一种利用Axin2抗体验证内源性蛋白标记的小鼠模型,证明Axin2可作为Wnt活性报告基因。抗体应用于检测基因编辑后Axin2的表达模式,助力发育生物学研究。

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这些文献涵盖了Axin2抗体在分子机制、癌症研究和转基因模型中的应用。如需具体期刊信息或年份,可进一步补充。

背景信息

The Axin 2 antibody is a critical tool for studying the Wnt/β-catenin signaling pathway, a conserved pathway regulating embryonic development, tissue homeostasis, and cancer. Axin 2 (Axis inhibition protein 2) acts as a scaffold protein in the β-catenin destruction complex, facilitating phosphorylation and degradation of β-catenin. Unlike Axin 1. Axin 2 is itself a transcriptional target of Wnt signaling, creating a negative feedback loop to modulate pathway activity. Researchers use Axin 2 antibodies to detect protein expression levels in techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate Axin 2's roles in developmental processes, stem cell regulation, and diseases such as colorectal cancer, hepatocellular carcinoma, and fibrosis. Axin 2 dysregulation is linked to tumor progression, making its antibody valuable in cancer research and biomarker studies. Commercial Axin 2 antibodies are typically raised against specific epitopes (e.g., human Axin2 C-terminal regions) and validated for species cross-reactivity (human, mouse, rat). Proper controls are essential due to potential cross-reactivity with Axin 1 or other proteins. Its utility extends to organoid research and regenerative medicine, reflecting Axin 2's importance in tissue repair and Wnt-driven pathologies.

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