| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OPMD; PAB2; PABII; PABP2; PABP-2 |
| Entrez GeneID | 8106 |
| WB Predicted band size | Calculated MW: 33 kDa; Observed MW: 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human PABPN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PABPN1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"Characterization of a Polyclonal Antibody Against Human PABPN1 Developed for Immunohistochemical Analyses"*
**作者**：Braun et al.
**摘要**：该研究开发了一种针对人源PABPN1的多克隆抗体，验证了其在免疫组化(IHC)和蛋白质印迹(Western blot)中的特异性，证明其可用于检测正常和病理状态下(如眼咽型肌营养不良，OPMD)的PABPN1蛋白表达及定位。
2. **文献名称**：*"Nuclear Aggregation of Polyadenylation RNA-Binding Proteins in Oculopharyngeal Muscular Dystrophy"*
**作者**：Tavanez et al.
**摘要**：本文利用PABPN1抗体研究OPMD患者中突变PABPN1蛋白的核内聚集现象，发现抗体可特异性识别异常聚集的蛋白包涵体，揭示了突变导致RNA代谢紊乱的分子机制。
3. **文献名称**：*"PABPN1 Functions in mRNA Polyadenylation and Stability: Insights from Knockdown and Antibody-Based Studies"*
**作者**：Fan et al.
**摘要**：通过抗体介导的蛋白定位和功能抑制实验，研究发现PABPN1在调控mRNA多聚腺苷酸化尾长度及稳定性中起关键作用，其缺失会导致基因表达异常和细胞核结构改变。
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注：以上文献为示例，实际文献可能需要根据具体数据库检索(如PubMed)。如需准确引用，建议通过关键词“PABPN1 antibody”、“PABPN1 immunohistochemistry”或“PABPN1 OPMD”查询最新研究。
Polyadenylate-binding protein nuclear 1 (PABPN1) is a critical RNA-binding protein involved in mRNA polyadenylation and stability. It facilitates the addition of poly(A) tails to nascent mRNA transcripts, ensuring proper nuclear export, translation, and stability. PABPN1 is predominantly localized in the nucleus, where it binds to poly(A) RNA and interacts with other components of the mRNA processing machinery. Structurally, it contains an N-terminal oligomerization domain and a C-terminal RNA-binding domain, enabling its role in regulating mRNA length and quality control.
Antibodies targeting PABPN1 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. Mutations in the PABPN1 gene, particularly expansions of a GCG triplet repeat encoding alanine, are linked to oculopharyngeal muscular dystrophy (OPMD), a rare autosomal dominant disorder characterized by muscle weakness and nuclear aggregates in affected cells. PABPN1 antibodies are widely used in research to detect pathological protein aggregates in OPMD models, investigate disease mechanisms, and evaluate therapeutic interventions.
These antibodies also aid in exploring PABPN1’s roles in cellular processes like RNA metabolism, stress responses, and aging. Commercial PABPN1 antibodies are typically validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry, enabling researchers to dissect its contributions to gene expression regulation and disease pathogenesis.
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