| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A; MRE11A; HNGS1; MRE11 |
| Entrez GeneID | 4361 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Mre11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Mre11抗体的代表性文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：*"Characterization of a Novel Monoclonal Antibody Against Human Mre11 for DNA Damage Studies"*
**作者**：Smith J. et al.
**摘要**：本研究开发了一种高特异性抗人Mre11单克隆抗体，验证了其在免疫印迹(Western blot)和免疫荧光中的适用性，证明其能有效检测细胞核内Mre11蛋白在DNA损伤反应中的定位变化。
2. **文献名称**：*"Mre11 Antibody Validation in Triple-Negative Breast Cancer Models"*
**作者**：Li X. et al.
**摘要**：通过免疫组化(IHC)和Co-IP实验，验证了一种商品化Mre11抗体在乳腺癌组织中的特异性，发现Mre11低表达与患者放疗抵抗显著相关，提示其作为预后标志物的潜力。
3. **文献名称**：*"Structural Insights into Mre11-Rad50 Complex Assembly Using Epitope-Specific Antibodies"*
**作者**：Kumar R. et al.
**摘要**：利用针对Mre11不同结构域的多克隆抗体，解析了Mre11-Rad50复合物的组装机制，揭示了其核酸内切酶活性调控的关键结构特征。
(注：以上文献为示例，实际引用需查询PubMed等数据库获取真实研究。)
The Mre11 antibody is a crucial tool in studying the Mre11 protein, a central component of the MRN complex (Mre11-Rad50-Nbs1), which plays vital roles in DNA damage response and repair. Mre11 exhibits endonuclease and exonuclease activities, facilitating resection of DNA double-strand breaks (DSBs) to promote homologous recombination repair (HRR) or non-homologous end joining (NHEJ). It also activates ATM kinase, coordinating cell cycle checkpoints and apoptosis. Dysregulation of Mre11 is linked to genomic instability, cancer predisposition, and disorders like ataxia-telangiectasia-like disorder (ATLD).
Mre11 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and interactions. Researchers rely on these antibodies to investigate DSB repair mechanisms, replication stress, and telomere maintenance. Specificity is validated using knockout cell lines or siRNA knockdown to ensure accurate detection. Commercial antibodies are typically raised in rabbits or mice, targeting conserved regions of human Mre11.
Applications extend to cancer research, where Mre11 expression correlates with therapeutic responses to radiation or PARP inhibitors. Additionally, these antibodies help explore Mre11’s role in meiosis, viral defense, and CRISPR-Cas9-induced DNA damage. Proper antibody validation remains critical, as off-target binding can complicate interpretations. Overall, Mre11 antibodies are indispensable for advancing our understanding of genome integrity and developing targeted therapies.
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