| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FP497; MGC15319; MGC42355; BID |
| Entrez GeneID | 637 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Bid |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Bid抗体的3篇经典文献及其摘要概括：
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1. **文献名称**：*Bid: A Novel BH3 Domain-Only Death Agonist*
**作者**：Wang, K., Yin, X.M., Chao, D.T., Milliman, C.L., Korsmeyer, S.J.
**摘要**：该研究首次鉴定Bid蛋白为仅含BH3结构域的促凋亡分子，揭示其在Fas/TNF-α受体介导的凋亡中被caspase-8激活为tBid，进而诱导线粒体释放细胞色素c。实验中通过特异性抗体验证了Bid的剪切及亚细胞定位。
2. **文献名称**：*Bid-deficient mice are resistant to Fas-induced hepatocellular apoptosis*
**作者**：Yin, X.M., Wang, K., Gross, A., et al.
**摘要**：通过构建Bid基因敲除小鼠，发现其肝细胞对Fas介导的凋亡具有抗性，证实Bid是死亡受体与线粒体凋亡通路的关键桥梁。研究利用Bid抗体分析蛋白表达缺失与表型关联。
3. **文献名称**：*Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis*
**作者**：Gross, A., Yin, X.M., Wang, K., et al.
**摘要**：阐明caspase-8对Bid的剪切生成活性片段tBid，后者易位至线粒体引发膜电位崩溃。实验通过Western blot和免疫荧光(依赖Bid抗体)证明tBid的线粒体靶向作用。
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这些研究奠定了Bid在凋亡中的核心地位，并展示了抗体在蛋白检测与功能解析中的关键作用。如需更近期文献或特定应用场景，可进一步补充。
Bid (BH3-interacting domain death agonist) is a pro-apoptotic protein belonging to the BCL-2 family, which plays a pivotal role in regulating mitochondrial apoptosis. It functions as a molecular bridge connecting extrinsic (death receptor-mediated) and intrinsic (mitochondria-mediated) apoptotic pathways. Inactive full-length Bid (22 kDa) resides in the cytosol until cleaved by caspase-8 or other proteases during apoptosis, generating truncated Bid (tBid, 15 kDa). This active form translocates to mitochondria, promoting cytochrome c release by interacting with BAX/BAK proteins, ultimately triggering caspase activation and cell death.
Bid antibodies are essential tools for detecting Bid expression, activation, and localization in apoptosis-related studies. They enable researchers to distinguish between full-length Bid and tBid via Western blot, monitor Bid phosphorylation status, and visualize subcellular redistribution during apoptosis using immunofluorescence. These antibodies are widely used in cancer research, neurodegenerative disease studies, and investigations of drug-induced apoptosis. Commercial Bid antibodies are typically raised against specific epitopes in human or mouse Bid, with validation across multiple applications. Understanding Bid dynamics through antibody-based assays has advanced therapeutic strategies targeting apoptosis pathways in diseases characterized by dysregulated cell survival.
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