| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | vesicle-associated membrane protein 2 (synaptobrevin 2); SYB2; VAMP-2 |
| Entrez GeneID | 6844 |
| WB Predicted band size | Calculated MW: 13 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VAMP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于VAMP2抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**：*Synaptic assembly of the brain in the absence of neurotransmitter secretion*
**作者**：Schoch, S., Deák, F., Königstorfer, A., et al.
**摘要**：本研究利用VAMP2基因敲除小鼠模型，通过VAMP2抗体的Western blot和免疫组化分析，证实了VAMP2在突触囊泡融合中的关键作用。结果显示，缺乏VAMP2会导致神经递质释放完全缺失，突触结构异常。
2. **文献名称**：*Molecular anatomy of a trafficking organelle*
**作者**：Takamori, S., Holt, M., Stenius, K., et al.
**摘要**：该研究通过VAMP2抗体的免疫印迹和质谱分析，系统定量了突触囊泡中的蛋白质组成。发现VAMP2是囊泡膜中丰度最高的SNARE蛋白之一，揭示了其在神经递质释放中的核心地位。
3. **文献名称**：*VAMP2 mediates neurotransmitter release via a syntaxin 1A-dependent pathway*
**作者**：Hua, Y., Scheller, R.H.
**摘要**：通过体外重构实验和VAMP2抗体的功能阻断实验，证明VAMP2与syntaxin 1A的相互作用是突触囊泡融合的必要条件。抗体抑制实验显示VAMP2在钙离子触发的膜融合中起直接作用。
---
以上文献均聚焦于VAMP2在突触传递中的功能，并利用其抗体进行蛋白质检测、定位或功能干预研究，适用于神经科学及细胞囊泡运输领域。
VAMP2 (vesicle-associated membrane protein 2), also known as synaptobrevin-2. is a key component of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex that mediates synaptic vesicle fusion and neurotransmitter release. As a transmembrane protein localized to synaptic vesicles, VAMP2 interacts with plasma membrane SNARE proteins syntaxin-1 and SNAP-25 to drive the exocytosis of neurotransmitters into the synaptic cleft. This process is critical for neuronal communication and synaptic plasticity.
VAMP2 antibodies are widely used tools in neuroscience research to study synaptic function, vesicle trafficking, and neurological disorders. They enable the detection, localization, and quantification of VAMP2 in tissues, cultured neurons, or transfected cell models. Aberrant VAMP2 expression or dysfunction has been implicated in conditions such as epilepsy, Parkinson’s disease, and diabetes, where insulin secretion relies on similar vesicle fusion mechanisms.
These antibodies are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity is ensured through knockout controls or peptide blocking assays. Some studies also employ VAMP2 antibodies to investigate SNARE complex assembly/disassembly dynamics or the impact of toxins like botulinum neurotoxin B, which cleaves VAMP2 to block neurotransmission. Overall, VAMP2 antibodies serve as essential probes for exploring synaptic biology and disease mechanisms linked to vesicular transport.
×
