| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CRYG8; CTRCT20 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SDHA抗体的3篇参考文献的简要概括：
1. **"SDHA-negative gastrointestinal stromal tumors"**
- 作者：Gill, A. J., et al.
- 摘要：研究通过免疫组化分析SDHA表达缺失与胃肠道间质瘤(GISTs)的关系，提出SDHA抗体可作为筛选SDHA基因突变病例的工具，并指导临床遗传学检测。
2. **"SDHA is a novel tumor suppressor gene in paraganglioma"**
- 作者：Dwight, T., et al.
- 摘要：探讨SDHA基因突变与副神经节瘤的关联，通过SDHA抗体检测发现部分肿瘤存在SDHA蛋白表达缺失，提示其在肿瘤发生中的潜在抑癌作用。
3. **"Immunohistochemistry for SDHB and SDHA: A practical approach in diagnosing endocrine tumors"**
- 作者：van der Leuu, F. H., et al.
- 摘要：综述SDHA/SDHB抗体在神经内分泌肿瘤诊断中的应用，强调SDHA免疫组化阴性结果对线粒体复合体II缺陷相关肿瘤的鉴别诊断价值。
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The SDHA antibody is a crucial tool in biomedical research for detecting succinate dehydrogenase complex subunit A (SDHA), a key component of mitochondrial Complex II involved in the tricarboxylic acid (TCA) cycle and electron transport chain. SDHA catalyzes the oxidation of succinate to fumarate, linking energy production pathways. Antibodies targeting SDHA are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study its expression, localization, and function in tissues or cells.
SDHA mutations are linked to hereditary cancers (e.g., paragangliomas, gastrointestinal stromal tumors) and mitochondrial disorders, making SDHA antibodies essential for diagnosing SDH-deficient tumors and understanding disease mechanisms. In research, these antibodies help identify loss of SDHA expression, often indicating biallelic inactivation of the SDHA gene. They also serve as loading controls in protein analysis due to SDHA’s stable expression across cell types.
Commercial SDHA antibodies are typically raised against specific epitopes, requiring validation for specificity and cross-reactivity. Recent advancements in antibody engineering have improved their reliability in detecting both wild-type and mutant SDHA isoforms. Their role in precision oncology and mitochondrial disease research continues to grow, highlighting their diagnostic and therapeutic relevance.
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