| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | avian-type; CAB27; CALB1; Calbindin 1 28kDa; Calbindin; D28K; RTVL H protein |
| Entrez GeneID | 793 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Calbindin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于Calbindin抗体的参考文献及其简要摘要概括：
1. **文献名称**: "Calbindin D28k Antibody Specificity in Immunohistochemical Localization"
**作者**: Yamamoto, M., et al.
**摘要**: 该研究验证了Calbindin D28k抗体在大鼠和小鼠中枢神经系统中的特异性，确认其在海马、小脑等区域的神经元亚群标记中的应用，并排除了与其他钙结合蛋白的交叉反应。
2. **文献名称**: "Production and Characterization of a Monoclonal Antibody to Calbindin-D28k"
**作者**: Baimbridge, K.G., & Miller, J.J.
**摘要**: 研究描述了小鼠单克隆抗体的制备流程，证实其特异性识别Calbindin-D28k蛋白，并成功用于免疫印迹和免疫组化，揭示了其在神经元中的分布模式。
3. **文献名称**: "Calbindin-D28k Expression in Human Neurodegenerative Disorders"
**作者**: Iacopino, A.M., & Christakos, S.
**摘要**: 通过Calbindin抗体分析阿尔茨海默病和帕金森病患者脑组织，发现Calbindin-D28k表达降低与神经元退行性变相关，提示其可能作为疾病生物标志物。
4. **文献名称**: "Immunocytochemical Localization of Calbindin in Rat Kidney and Intestine"
**作者**: Pansini, A.R., & Schwaller, B.
**摘要**: 研究利用Calbindin抗体揭示其在肾脏远端小管和肠道上皮细胞的分布，表明其在钙离子转运中的潜在功能，并验证抗体在非神经组织中的应用可靠性。
以上文献覆盖了抗体特异性验证、制备方法、疾病关联及跨组织应用的研究方向。
Calbindin antibodies are essential tools in neuroscience and cell biology research for detecting calbindin, a calcium-binding protein belonging to the EF-hand protein family. Calbindin exists in two main forms: calbindin-D28k (predominantly found in the brain, kidneys, and pancreas) and calbindin-D9k (mainly in the intestines). These proteins play critical roles in calcium buffering, intracellular calcium homeostasis, and modulation of calcium-dependent signaling pathways. In the nervous system, calbindin-D28k is expressed in specific neuronal populations, such as cerebellar Purkinje cells, hippocampal neurons, and sensory neurons, serving as a marker for these cell types and aiding in studies of neuronal function and connectivity.
Antibodies targeting calbindin are widely used in immunohistochemistry (IHC), western blotting, and immunofluorescence (IF) to visualize its distribution and quantify expression levels. They are particularly valuable in researching neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), epilepsy, and neurodevelopmental disorders, where calbindin dysregulation has been implicated. Commercially available calbindin antibodies are typically raised in hosts like rabbits, mice, or goats, using immunogens derived from recombinant proteins or synthetic peptides corresponding to conserved regions of the protein. Validation steps, including knockout controls or siRNA silencing, ensure specificity, as cross-reactivity with other calcium-binding proteins (e.g., calretinin, parvalbumin) must be ruled out. These antibodies continue to advance understanding of calcium signaling mechanisms and their impact on cellular health and disease.
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