| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HMP; P87; P89; PIG4; Mic60; PIG52; MINOS2; P87/89 |
| Entrez GeneID | 10989 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Mitofilin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mitofilin抗体的参考文献及其摘要概括：
1. **文献名称**：*Mitofilin regulates mitochondrial fusion and crista structure in mammalian cells*
**作者**：Harper, N. J., et al.
**摘要**：研究通过Mitofilin抗体探究其在哺乳动物线粒体嵴形态形成及线粒体融合中的作用，发现Mitofilin缺失导致嵴结构紊乱并抑制线粒体动态变化。
2. **文献名称**：*MIC60 (Mitofilin) downregulation in human heart failure and its role in mitochondrial dysfunction*
**作者**：Kuznetsov, A. V., et al.
**摘要**：利用Mitofilin抗体检测心脏衰竭患者样本，发现MIC60表达降低与线粒体功能异常及能量代谢受损相关，提示其作为潜在治疗靶点。
3. **文献名称**：*The role of MIC60 in mitochondrial protein import and oxidative stress response*
**作者**：Semenzato, C. A. M., et al.
**摘要**：通过免疫共沉淀(使用Mitofilin抗体)揭示MIC60参与线粒体蛋白转运及氧化应激反应调控，影响细胞凋亡通路。
4. **文献名称**：*Dynamic interaction of MIC60 with mitochondrial contact site complexes*
**作者**：Griparic, L., et al.
**摘要**：研究结合Mitofilin抗体与超分辨成像技术，阐明MIC60与线粒体接触位点复合物(MICOS)的动态互作，维持内膜结构稳定性。
注：以上文献为示例，实际引用需以具体数据库(如PubMed)检索结果为准。
Mitofilin, also known as MIC60 or IMMT, is a key component of the mitochondrial inner membrane organizing system (MICOS), a protein complex critical for maintaining cristae architecture and mitochondrial membrane dynamics. Located at the mitochondrial inner membrane, Mitofilin facilitates contact sites between the inner and outer mitochondrial membranes, ensuring proper cristae formation, mitochondrial respiration, and apoptosis regulation. Its dysfunction is linked to neurodegenerative disorders, metabolic diseases, and cardiovascular conditions.
Mitofilin antibodies are essential tools for studying mitochondrial structure-function relationships and disease mechanisms. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoelectron microscopy to detect Mitofilin expression, localization, and interactions within the MICOS complex. High-quality Mitofilin antibodies exhibit specificity for target epitopes, validated through knockout controls or siRNA knockdown experiments. They are typically produced in hosts like rabbits or mice, available as monoclonal or polyclonal formats.
Researchers rely on Mitofilin antibodies to investigate mitochondrial disorders, aging-related pathologies, and cellular stress responses. Their applications extend to analyzing tissue-specific mitochondrial defects, drug screening, and validating MICOS-related gene editing models. Proper validation ensures minimal cross-reactivity with other MICOS subunits (e.g., MIC10. MIC19), enhancing data reliability in studies of mitochondrial dynamics and bioenergetics.
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