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Rabbit Polyclonal ARAF Antibody

  • 中文名： A RAF抗体
  • 别    名： ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
货号: IPDX22872
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
Entrez GeneID369
WB Predicted band sizeCalculated MW: 68 kDa; Observed MW: 68 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human A-RAF
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于A-RAF抗体的3篇虚构参考文献示例(仅供参考，非真实文献)：

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1. **文献名称**：*"A Novel Monoclonal Antibody Targeting A-RAF for Functional Studies in MAPK Signaling"*

**作者**：Thompson L, et al.

**摘要**：本研究报道了一种高特异性抗A-RAF单克隆抗体的开发，通过免疫印迹和免疫荧光验证其与A-RAF的结合能力，并证明其在抑制MAPK通路活性中的作用，为A-RAF功能研究提供了新工具。

2. **文献名称**：*"A-RAF Expression Profiling in Lung Adenocarcinoma Using a Phospho-Specific Antibody"*

**作者**：Sato K, et al.

**摘要**：利用磷酸化特异性A-RAF抗体(pSer299)，分析了肺癌组织中A-RAF的激活状态，发现其磷酸化水平与EGFR突变呈正相关，提示A-RAF可能作为EGFR驱动肿瘤的潜在生物标志物。

3. **文献名称**：*"Antibody-Based Screening Reveals A-RAF's Role in Neuronal Differentiation"*

**作者**：Martinez R, et al.

**摘要**：通过A-RAF特异性抗体敲低实验，揭示A-RAF通过调控MEK/ERK信号通路促进神经干细胞分化，为神经发育疾病提供了机制见解。

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**备注**：

- 如需真实文献，建议在PubMed或Google Scholar中搜索关键词：`A-RAF antibody`、`ARAF kinase antibody`，并筛选近年研究。

- 部分商业抗体公司(如Cell Signaling Technology #4432、Abcam ab137435)会提供其抗体的验证文献，可直接参考产品说明书中的引用。

背景信息

The ARAF (A-Raf) antibody is a research tool targeting the A-Raf protein, a member of the Raf kinase family (including A-Raf, B-Raf, and C-Raf/Raf1) within the MAPK/ERK signaling pathway. This serine/threonine kinase plays a regulatory role in cell proliferation, differentiation, and survival by transmitting signals from membrane receptors (e.g., Ras) to downstream effectors like MEK and ERK. While less studied than B-Raf (notably due to B-Raf's frequent mutations in cancers), A-Raf shares structural homology and overlapping functions but exhibits distinct tissue-specific expression and weaker kinase activity. ARAF antibodies are critical for detecting A-Raf expression, phosphorylation status, or localization in cellular studies. Dysregulation of A-Raf has been implicated in certain cancers (e.g., melanoma, colorectal), developmental disorders, and drug resistance mechanisms, particularly in contexts where B-Raf inhibitors drive paradoxical pathway activation. Research using ARAF antibodies aims to clarify its unique biological roles, compensatory signaling in Raf-isoform knockout models, and therapeutic targeting strategies. Commercial ARAF antibodies are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding both basic and translational studies of MAPK pathway dynamics.

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